a Department of Ophthalmology , The Affiliated Hospital of Qingdao University , Qingdao , Shandong Province , China.
b Department of Vascular Surgery , Huangdao Branch of the Affiliated Hospital of Qingdao University , Qingdao , Shandong Province , China.
Curr Eye Res. 2019 Jul;44(7):716-725. doi: 10.1080/02713683.2019.1584321. Epub 2019 Mar 29.
: Pannexin 1 channels are deemed to play important roles in inflammation. However, there is limited information regarding their roles in fungal infection diseases, especially fungal keratitis. This study aimed to investigate the role of pannexin 1 channels in () keratitis. : Mouse models or immortalized human corneal epithelial cells (HCECs) were infected with or without for given time. The expression of pannexin 1 channels was tested by qPCR, western blot and immunofluorescence staining. Mice of keratitis were pretreated with carbenoxolone (CBX) or 2'(3')-O-(4-Benzoylbenzoyl) adenosine-5'-triphosphate (BzATP) to block or activate the opening of pannexin 1 channels respectively. The clinical score was recorded. Cornea tissues were examined for the downstream signals of pannexin 1 channels, including NLRP3, Caspase-1 and IL-1β, and myeloperoxidase (MPO) by PCR and ELISA. Data were analyzed with commercial data analysis software and a < 0.05 was considered to be statistically significant. : Upon infection, pannexin 1 expression increased at both the mRNA and the protein levels in mice corneas (< 0.05, n = 3). Immunofluorescence indicated that pannexin 1 channels were mainly located in the corneal epithelial layer, and they were upregulated after infection. In vitro, the same tendency was found at the mRNA and the protein levels in HCECs (< 0.05, n = 8). In mouse model, blockage of pannexin 1 channels by CBX caused more severely keratitis. The downstream signals of pannexin 1 channels (NLRP3/Caspase-1/IL-1β) and MPO were down-regulated. Whereas activation the opening of pannexin 1 channels by BzATP reduced corneal infection with increased expression of Caspase-1 and IL-1β. : Pannexin 1 channels play important roles in the regulation of progression and leucocytes aggregation during corneal infection via the NLRP3/Caspase-1/IL-β pathway.
: 质膜通道蛋白 1 （pannexin 1 ）通道被认为在炎症中发挥重要作用。然而，关于其在真菌感染性疾病中的作用，特别是真菌性角膜炎，信息有限。本研究旨在探讨 pannexin 1 通道在（）角膜炎中的作用。 : 用或不用（）感染小鼠模型或永生化人角膜上皮细胞（HCECs ）一定时间。通过 qPCR 、 Western blot 和免疫荧光染色检测 pannexin 1 通道的表达。用 carbenoxolone （CBX ）或 2' （3' ） -O- （4- 苯甲酰基苯甲酰基）腺苷-5'-三磷酸（BzATP ）预处理（）角膜炎小鼠，分别阻断或激活 pannexin 1 通道的开放。记录临床评分。通过 PCR 和 ELISA 检测角膜组织中 pannexin 1 通道的下游信号，包括 NLRP3 、 Caspase-1 和 IL-1β以及髓过氧化物酶（MPO ）。用商业数据分析软件分析数据， < 0.05 认为具有统计学意义。 : 在（）感染后，小鼠角膜中 pannexin 1 的表达在 mRNA 和蛋白水平均增加（< 0.05 ， n = 3 ）。免疫荧光显示 pannexin 1 通道主要位于角膜上皮层，感染后上调。在体外，HCECs 中也发现了同样的趋势，在 mRNA 和蛋白水平上均上调（< 0.05 ， n = 8 ）。在小鼠模型中，CBX 阻断 pannexin 1 通道导致更严重的角膜炎。pannexin 1 通道的下游信号（NLRP3/Caspase-1/IL-1β）和 MPO 下调。而 BzATP 激活 pannexin 1 通道可减少角膜感染，增加 Caspase-1 和 IL-1β的表达。 : pannexin 1 通道通过 NLRP3/Caspase-1/IL-β 途径在角膜（）感染过程中调节进展和白细胞聚集，发挥重要作用。
