Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. China.
Key Laboratory of Smart Drug Delivery, Ministry of Education and PLA, Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, P. R. China.
J Sep Sci. 2019 May;42(9):1794-1815. doi: 10.1002/jssc.201800952. Epub 2019 Apr 14.
Berberine alkaloids, a group of protoberberine alkaloids under the classification of isoquinoline alkaloids, include berberine, coptisine, palmatine, columbamine, dehydrocorydaline, jatrorrhizine, and epiberberine from natural sources. Studies have shown that berberine alkaloids have various pharmacological functions, such as antibacterial, antiviral, blood pressure-lowering, hypoglycaemic, antiarrhythmia, and anticancer effects. Therefore, it is worthwhile to develop analytical methods to investigate the pharmacokinetics and activity mechanisms of berberine alkaloids and to study berberine alkaloids more comprehensively. Current analytical methods for berberine alkaloids include liquid chromatography, thin-layer chromatography, ultraviolet spectroscopy, capillary electrophoresis, and gas chromatography. The most widely used detection method is mass spectrometry. In order to provide a systematic and comprehensive summary and to serve as a reference for the future pharmacokinetics studies and analysis of berberine alkaloids, analytical methods for natural berberine alkaloids that have been used in the past ten years are reviewed here.
小檗碱生物碱是异喹啉生物碱分类下的一类原小檗碱生物碱，包括天然来源的小檗碱、黄连碱、巴马汀、小檗胺、脱氢紫堇碱、药根碱和表小檗碱。研究表明，小檗碱生物碱具有多种药理作用，如抗菌、抗病毒、降压、降血糖、抗心律失常和抗癌作用。因此，开发分析方法来研究小檗碱生物碱的药代动力学和作用机制，并更全面地研究小檗碱生物碱是值得的。目前用于小檗碱生物碱的分析方法包括液相色谱法、薄层色谱法、紫外光谱法、毛细管电泳法和气相色谱法。最常用的检测方法是质谱法。为了提供一个系统和全面的总结，并为小檗碱生物碱的未来药代动力学研究和分析提供参考，本文综述了过去十年中用于天然小檗碱生物碱的分析方法。
