用于检测二乙酰氧基藨草镰刀菌烯醇的灵敏酶联免疫吸附测定法的开发。
Development of a sensitive enzyme-linked immunosorbent assay for the detection of diacetoxyscirpenol.
作者信息
Klaffer U, Märtlbauer E, Terplan G
机构信息
Lehrstuhl für Hygiene und Technologie der Milch, München, F.R.G.
出版信息
Int J Food Microbiol. 1988 Feb;6(1):9-17. doi: 10.1016/0168-1605(88)90079-7.
An enzyme-linked immunosorbent assay (ELISA) for the detection of diacetoxyscirpenol (DAS) was developed. Antibodies against DAS were obtained after immunization of rabbits with DAS-hemiglutarate-human serum albumin (DAS-HG-HSA). After an immunization period of 6 weeks a sufficient level of specific antibodies was reached. A DAS-hemisuccinate-horseradish peroxidase-conjugate (DAS-HS-HRP) was prepared by an ester method and used as enzyme-labelled toxin in the competitive assay. The detection limit for DAS was about 10 pg/ml. The relative cross reactivity of the assay was 597.5, 5.2, 100.0, 2.5, and 1.5% for 3 alpha-acetyl-DAS, DAS, T-2 toxin, neosolaniol, and 15-acetoxyscirpenol, respectively.
开发了一种用于检测二乙酰氧基雪腐镰刀菌烯醇（DAS）的酶联免疫吸附测定（ELISA）。用DAS - 半戊二酸 - 人血清白蛋白（DAS - HG - HSA）免疫兔子后获得了抗DAS抗体。免疫6周后达到了足够水平的特异性抗体。通过酯法制备了DAS - 半琥珀酸 - 辣根过氧化物酶缀合物（DAS - HS - HRP），并将其用作竞争测定中的酶标记毒素。DAS的检测限约为10 pg/ml。该测定对3α - 乙酰 - DAS、DAS、T - 2毒素、新茄病镰刀菌烯醇和15 - 乙酰氧基雪腐镰刀菌烯醇的相对交叉反应率分别为597.5%、5.2%、100.0%、2.5%和1.5%。
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