抗T-2毒素抗体的产生。
Production of antibody against T-2 toxin.
作者信息
Chu F S, Grossman S, Wei R D, Mirocha C J
出版信息
Appl Environ Microbiol. 1979 Jan;37(1):104-8. doi: 10.1128/aem.37.1.104-108.1979.
Abstract
Antibody against T-2 toxin was obtained after immunization of rabbits with bovine serum albumin-T-2 hemisuccinate conjugate. The antibody had greatest binding efficiency for T-2 toxin, less efficiency for HT-2, and least for T-2 triol. Cross-reaction of antibody with neosolaniol, T-2 tetraol, and 8-acetyl-neosolaniol was very weak. Diacetoxyscirpenol, trichodermin, vomitoxin, and verrucarin A essentially gave no cross-reaction with the antibody. The sensitivity of the binding assay for T-2 toxin detection was in the range of 1 to 20 ng per assay. Detailed methods for the preparation of the conjugate and the production of immune serum and methods for antibody determination are described.
摘要
用牛血清白蛋白 - T - 2半琥珀酸酯共轭物免疫兔子后获得了抗T - 2毒素抗体。该抗体对T - 2毒素的结合效率最高，对HT - 2的效率较低，对T - 2三醇的效率最低。抗体与新茄病镰刀菌烯醇、T - 2四醇和8 - 乙酰新茄病镰刀菌烯醇的交叉反应非常弱。二醋酸雪腐镰刀菌烯醇、木霉菌素、呕吐毒素和疣孢菌素A与该抗体基本无交叉反应。T - 2毒素检测结合试验的灵敏度为每次试验1至20纳克。文中描述了共轭物制备、免疫血清生产的详细方法以及抗体测定方法。
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