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俄勒冈州、华盛顿州和加利福尼亚州由新壳梭孢属(Neofabraea spp.)引起的梨靶斑病病因

Etiology of Bull's Eye Rot of Pear Caused by Neofabraea spp. in Oregon, Washington, and California.

作者信息

Henriquez Jose L, Sugar David, Spotts Robert A

机构信息

Oregon State University Southern Oregon Research and Extension Center, Medford 97502.

Mid-Columbia Agricultural Research and Extension Center, Hood River, OR 97031.

出版信息

Plant Dis. 2004 Oct;88(10):1134-1138. doi: 10.1094/PDIS.2004.88.10.1134.

Abstract

A collection of Neofabraea isolates from pear fruit grown in Oregon, Washington, and California was screened with species-specific primers in a multiplex polymerase chain reaction (PCR). Neofabraea alba was identified most frequently in samples from Oregon and California, whereas N. perennans was found most frequently in samples from Washington. N. alba also was identified from tissue of small cankers and pruning stubs on pear trees using PCR. Bull's eye rot pathogens were isolated from fruit of nine different European pear cultivars, Asian pear, and quince. Overall, N. alba was the most prevalent species in 2001 whereas N. perennans was more prevalent in 2002. An undescribed species of Neofabraea was identified in samples from Medford, OR that corresponds to a species previously found by others using molecular methods. This information increases the known geographic distribution of this undescribed species.

摘要

利用多重聚合酶链反应(PCR)中的物种特异性引物,对从俄勒冈州、华盛顿州和加利福尼亚州种植的梨果中分离得到的一批新壳梭孢菌菌株进行了筛选。在俄勒冈州和加利福尼亚州的样本中,最常鉴定出白色新壳梭孢菌,而在华盛顿州的样本中,最常发现多年生新壳梭孢菌。还通过PCR从梨树小溃疡和修剪残端的组织中鉴定出了白色新壳梭孢菌。从9个不同的欧洲梨品种、亚洲梨和榅桲的果实中分离出了靶心腐病菌。总体而言,白色新壳梭孢菌在2001年是最普遍的物种,而多年生新壳梭孢菌在2002年更为普遍。在俄勒冈州梅德福的样本中鉴定出了一种未描述的新壳梭孢菌物种,它与其他人之前使用分子方法发现的一个物种相对应。这一信息增加了这种未描述物种已知的地理分布范围。

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