School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, People's Republic of China.
School of Biomedical Engineering, Xinxiang Neurosense and Control Engineering Technology Research Center, Xinxiang Key Lab of Biomedical Information Research, Xinxiang Medical University, Xinxiang, Henan, 453003, People's Republic of China.
Mikrochim Acta. 2019 Feb 22;186(3):201. doi: 10.1007/s00604-019-3320-1.
This article describes the development of several nanoconjugates composed of cobalt (III) oxyhydroxide and DEASPI/βCDP, where DEASPI stands for the dye trans-4-[p-(N,N-diethylamino)styryl]-N-methylpyridinium, and βCDP stands for β-cyclodextrin. The material enables sensitive fluorometric detection and 3D imaging of ascorbic acid (AA) in biological samples. A nanomicelle composed of DEASPI and βCDP was prepared to act as a two-photon absorbance (TPA) nanofluorophore with desirable two-photon-sensitized fluorescence, high penetration depth, and excellent cell-permeability). The CoOOH nanoflakes were placed on the surface of the nanomicelle to act as both a quencher of fluorescence and as the recognition unit for AA. In the presence of AA, the CoOOH nanoflakes are reduced to Co (II), and this triggers the recovery of fluorescence. This new nanoprobe exhibits amplified two-photon fluorescence (excitation at 840 nm; emission at 565 nm), high sensitivity, and good selectivity. In-vitro imaging of endogenous AA was demonstrated in living HeLa cells. It was also employed to 3D imaging of exogenous AA in tissue by two-photon excitation microscopy to a depth of up to 320 μm. In our perception, this nanoprobe represents a valuable tool for elucidating the roles of AA in biochemical and clinical studies. Graphical abstract Schematic presentation of the preparation of a novel Poly β-Cyclodextrin/TPdye conjugated with cobalt oxyhydroxide nanoplatform and its application for high sensitive and two-photon 3D imaging of ascorbic acid (AA) in living cells and deep tissues.
本文描述了几种由钴（III）氢氧化物和 DEASPI/βCDP 组成的纳米缀合物的开发，其中 DEASPI 代表染料反式-4-[p-(N,N-二乙基氨基)苯乙烯基]-N-甲基吡啶鎓，βCDP 代表β-环糊精。该材料能够实现生物样品中抗坏血酸（AA）的灵敏荧光检测和 3D 成像。制备了由 DEASPI 和 βCDP 组成的纳米胶束，用作具有理想双光子吸收（TPA）的纳米荧光团，具有双光子敏化荧光、高穿透深度和优异的细胞通透性。将 CoOOH 纳米薄片放置在纳米胶束的表面上，作为荧光的猝灭剂和 AA 的识别单元。在 AA 的存在下，CoOOH 纳米薄片被还原为 Co（II），这触发了荧光的恢复。这种新的纳米探针表现出放大的双光子荧光（激发波长 840nm；发射波长 565nm）、高灵敏度和良好的选择性。在活 HeLa 细胞中进行了内源性 AA 的体外成像。还将其用于双光子激发显微镜对组织中外源性 AA 的 3D 成像，深度可达 320μm。在我们看来，这种纳米探针代表了一种有价值的工具，可用于阐明 AA 在生化和临床研究中的作用。
