Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China.
Environ Sci Pollut Res Int. 2019 Apr;26(11):11279-11287. doi: 10.1007/s11356-019-04519-1. Epub 2019 Feb 22.
Chlordecone (CLD), also named Kepone, is a synthetic organochlorine pesticide. As one of the common persistent organic pollutants (POPs) in nature, CLD has a profound impact on the environment and human health. The study aims to investigate the reproductive toxicity effects of CLD on male Caenorhabditis elegans and on progeny. L1-stage male nematodes were exposed to the control group (M9 solution) and four dose groups (0.02, 0.2, 2, and 20 μg/L). After exposure for 48 h, the male nematodes were picked to mating experiment and progeny experiment that the number of progeny and the time of observation in male parent and in F1 generation were counted; the number of germ cells and the number of sperm in the meiotic division of male nematodes were counted by staining with dimercaptophenyl hydrazine (DAPI), and the nematode gland area was observed under the bright field of the microscope. In male nematodes, the results showed that a number of progeny were 351.20 ± 31.40, 321.60 ± 24.70, 307.30 ± 19.30, 240.10 ± 27.60, and 227.90 ± 22.70 (P < 0.05); the generation times were 55.80 ± 1.95 h, 56.40 ± 1.60 h, 56.70 ± 0.92 h, 60.80 ± 0.95 h, and 69.60 ± 1.97 h (P < 0.05); relative areas of gonad were (99.80 ± 6.27)%, (93.00 ± 1.70)%, (85.00 ± 1.70)%, (70.70 ± 9.81)%, and (60.00 ± 5.23)% (P < 0.05); DAPI staining results showed the number of germ cells in meiosis area were 191.00 ± 10.97, 181.10 ± 15.56, 177.00 ± 9.20, 147.50 ± 10.56, and 139.30 ± 23.79 (P < 0.05); the sperm numbers were 335.60 ± 21.31, 308.60 ± 19.60, 306.00 ± 11.23, 260.10 ± 27.41, and 255.00 ± 3.72 (P < 0.05). In the F1 generation, the progeny numbers were 328.10 ± 22.28, 167.50 ± 15.30, 150.00 ± 13.65, 131.30 ± 18.40, and 130.20 ± 16.17 (P < 0.05); the generation times were 55.50 ± 2.36, 71.10 ± 0.97, 70.90 ± 0.52, 74.10 ± 2.07, and 73.90 ± 1.35 h (P < 0.05). The groups are grouped in order as M9 solution, 0.02, 0.2, 2, and 20 μg/L. The results revealed that CLD caused decrease in progeny number, relative area of gonad, number of germ cells, and sperm number and prolonged the generation time in the male nematode. In offspring grown up without CLD, the effect of CLD on generation time and sperm number can still be observed on offspring. In conclusion, CLD induces male nematode reproductive toxicity and causes defects in offspring.
十氯酮（CLD），又名开蓬，是一种合成有机氯农药。作为自然界中常见的持久性有机污染物（POPs）之一，CLD 对环境和人类健康有深远的影响。本研究旨在探讨 CLD 对雄性秀丽隐杆线虫及其后代的生殖毒性作用。将 L1 期雄性线虫暴露于对照组（M9 溶液）和四个剂量组（0.02、0.2、2 和 20μg/L）中。暴露 48 小时后，雄性线虫被挑选出来进行交配实验和后代实验，记录后代数量和雄性亲本及 F1 代的观察时间；用二巯基苯肼（DAPI）染色计数雄性线虫减数分裂中的生殖细胞和精子数量，并在明场显微镜下观察线虫腺区。在雄性线虫中，结果显示后代数量分别为 351.20±31.40、321.60±24.70、307.30±19.30、240.10±27.60 和 227.90±22.70（P<0.05）；世代时间分别为 55.80±1.95 h、56.40±1.60 h、56.70±0.92 h、60.80±0.95 h 和 69.60±1.97 h（P<0.05）；性腺相对面积分别为（99.80±6.27）%、（93.00±1.70）%、（85.00±1.70）%、（70.70±9.81）%和（60.00±5.23）%（P<0.05）；DAPI 染色结果显示减数分裂区生殖细胞数量分别为 191.00±10.97、181.10±15.56、177.00±9.20、147.50±10.56 和 139.30±23.79（P<0.05）；精子数量分别为 335.60±21.31、308.60±19.60、306.00±11.23、260.10±27.41 和 255.00±3.72（P<0.05）。在 F1 代中，后代数量分别为 328.10±22.28、167.50±15.30、150.00±13.65、131.30±18.40 和 130.20±16.17（P<0.05）；世代时间分别为 55.50±2.36 h、71.10±0.97 h、70.90±0.52 h、74.10±2.07 h 和 73.90±1.35 h（P<0.05）。各实验组分别为 M9 溶液、0.02、0.2、2 和 20μg/L。结果表明，CLD 导致后代数量减少、性腺相对面积减小、生殖细胞和精子数量减少以及世代时间延长。在没有 CLD 的雄性线虫后代中，仍能观察到 CLD 对世代时间和精子数量的影响。综上所述，CLD 诱导雄性线虫生殖毒性，并导致后代出现缺陷。
