Ozpinar Hulya, Ozpinar Necati, Karakus Savas
Department of Pharmaceutical Botany, Faculty of Pharmacy, Cumhuriyet University, Sivas, Turkey.
Department of Parasitology, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
Pharmacogn Mag. 2017 Jul;13(Suppl 2):S380-S384. doi: 10.4103/0973-1296.210164. Epub 2017 Jul 11.
Grapes and their products are known to have been used for the treatment of diseases throughout history.
It was aimed to investigate the effects of Erzincan Cimin grapes on an organism model of N2 wild type and BS913 strains with gonad cancer.
The effects of methanol extracts of the skin and seeds of Erzincan Cimin grapes were examined separately on N2 wild type and an effect was determined on lifespan. By applying GS-MS analysis, a potential agent substance was determined in the skin and seed methanol extracts. This substance was purchased and the effects of this substance were investigated on lifespan and fertility in BS913 strains with gonad cancer. In addition, the effects on young subjects exposed to this agent substance in L1 form were investigated.
Grape seed and skin methanol extract was observed to prolong the lifespan most at a dose of 10 mg/100 mL. Lifespan was determined to be at a maximum in a gonad cancer organism model with benzothiazol at a dose of 50 ppm. At the same dose, positive effects were determined on the fertility of strains with cancer. When the effects of benzothiazol were examined on young L1 forms, an evident retardation of growth was determined at doses of 10, 50, and 100 ppm.
Owing to anti-carcinogenic effects of benzothiazol and benzothiazol-derived substances, they can be considered as agent substances in academic studies related to cancer.
The effects of methanol extracts of the skin and seeds of Erzincan Cimin grapes were examined on N2 wild type and an effect was determined on lifespanThrough GS-MS analysis, benzothiazol was determined in the skin methanol extractsBenzothiazol was purchased and the effects of this substance were investigated on lifespan and fertility in BS913 strains with gonad cancerThe effects on young subjects exposed to benzothiazol in L1 formGrape seed, skin methanol extract, and benzothiazol was observed to prolong the lifespanPositive effects were determined on the fertility of gonad cancer strains. GC-MS: gas chromatography and mass spectrometry; : ; NGM: Nematode growth medium; : ; FUDR: Fluorodeoxyuridine; LDL: Low-density lipoprotein.
众所周知，葡萄及其制品在历史上一直被用于治疗疾病。
旨在研究埃尔津詹奇明葡萄对N2野生型和患有性腺癌的BS913菌株的生物体模型的影响。
分别检测了埃尔津詹奇明葡萄皮和籽的甲醇提取物对N2野生型的影响，并测定了对寿命的影响。通过气相色谱-质谱联用（GS-MS）分析，在葡萄皮和籽的甲醇提取物中确定了一种潜在的活性物质。购买了该物质，并研究了其对患有性腺癌的BS913菌株的寿命和生育能力的影响。此外，还研究了以L1形式接触该活性物质的年轻个体所受的影响。
观察到葡萄籽和葡萄皮甲醇提取物在剂量为10mg/100mL时最能延长寿命。在患有性腺癌的生物体模型中，剂量为50ppm的苯并噻唑使寿命达到最长。在相同剂量下，对癌症菌株的生育能力有积极影响。当检测苯并噻唑对年轻L1形式的影响时，在10、50和100ppm剂量下确定有明显的生长迟缓。
由于苯并噻唑及其衍生物质的抗癌作用，它们可被视为癌症相关学术研究中的活性物质。
检测了埃尔津詹奇明葡萄皮和籽的甲醇提取物对N2野生型的影响，并测定了对寿命的影响。通过GS-MS分析，在葡萄皮甲醇提取物中确定了苯并噻唑。购买了苯并噻唑，并研究了其对患有性腺癌的BS913菌株的寿命和生育能力的影响。研究了以L1形式接触苯并噻唑的年轻个体所受的影响。观察到葡萄籽、葡萄皮甲醇提取物和苯并噻唑能延长寿命。对性腺癌菌株的生育能力有积极影响。GC-MS：气相色谱和质谱联用；：；NGM：线虫生长培养基；：；FUDR：氟脱氧尿苷；LDL：低密度脂蛋白。
