Yu Zhenyang, Sun Guohua, Liu Yanjun, Yin Daqiang, Zhang Jing
State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China.
College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang 314001, PR China; Department of Civil and Environmental Engineering, University of Ulsan, Nam Gu, Ulsan 680-748, South Korea.
Ecotoxicol Environ Saf. 2017 Jan;135:312-318. doi: 10.1016/j.ecoenv.2016.10.017. Epub 2016 Oct 19.
Trans-generational effects are increasingly used to indicate long-term influences of environmental pollutants. However, such studies can be complex and yield inconclusive results. In this study, the trans-generational effects of sulfamethoxazole (SMX) on Caenorhabditis elegans on lifespan, reproduction and population growth were tested for 7 consecutive generations, which included gestating generation (F0), embryo-exposed generation (F1), germline-exposed generation (F2), the first non-exposed generation (F3) and the three following generations (F4-F6). Results showed that lifespan was significantly affected by embryo exposure (F1) at 400µm SMX with a value as low as 47% of the control. The reproduction (a total brood size as 49% of the control) and population growth (81% of the control) were significantly affected in germline exposure (F2). Lifespan and reproduction were severely inhibited in non-exposed generations, confirming the real trans-generational effects. Notably, initial reproduction and reproduction duration showed opposite generation-related changes, indicating their interplay in the overall brood size. The population growth rate was well correlated with median lethal time, brood size and initial reproduction, which indicated that the population would increase when the nematodes lived longer and reproduced more offspring within shorter duration.
跨代效应越来越多地被用于表明环境污染物的长期影响。然而，此类研究可能很复杂，结果也尚无定论。在本研究中，连续7代测试了磺胺甲恶唑（SMX）对秀丽隐杆线虫寿命、繁殖和种群增长的跨代效应，这7代包括妊娠代（F0）、胚胎暴露代（F1）、生殖系暴露代（F2）、第一代未暴露代（F3）以及随后的三代（F4 - F6）。结果表明，在400µm磺胺甲恶唑作用下，胚胎暴露（F1）显著影响寿命，其值低至对照组的47%。生殖系暴露（F2）显著影响繁殖（总产卵量为对照组的49%）和种群增长（为对照组的81%）。在未暴露代，寿命和繁殖受到严重抑制，证实了真正的跨代效应。值得注意的是，初始繁殖和繁殖持续时间呈现出与代相关的相反变化，表明它们在总产卵量中相互作用。种群增长率与半数致死时间、产卵量和初始繁殖密切相关，这表明当线虫寿命更长且在更短时间内繁殖更多后代时，种群将会增加。
