Laboratory for Biology of Secondary Metabolism, Institute of Microbiology of the Academy of Sciences of the Czech Republic, BIOCEV, Průmyslová 595, 252 50 Vestec, Czech Republic.
Laboratory for Biology of Secondary Metabolism, Institute of Microbiology of the Academy of Sciences of the Czech Republic, BIOCEV, Průmyslová 595, 252 50 Vestec, Czech Republic.
J Glob Antimicrob Resist. 2019 Mar;16:251-253. doi: 10.1016/j.jgar.2019.02.005. Epub 2019 Feb 20.
The aim of this study was to analyse the DNA sequences of three teicoplanin-resistant Staphylococcus epidermidis isolates collected from patients not previously treated with glycopeptide antibiotics.
The minimum inhibitory concentrations (MICs) of 12 antibiotics, including teicoplanin and vancomycin, were determined by the broth microdilution method. Genomic DNA was isolated, was sequenced by HiSeqX paired-end sequencing and was assembled into draft genome sequences using MyPro pipeline.
Analysis of the draft genome sequences demonstrated that the teicoplanin-resistant S. epidermidis isolates belonged to multilocus sequence typing (MLST) sequence types ST5 and ST87 and encoded multiple antimicrobial resistance genes, including the methicillin resistance gene mecA.
This report highlights the risk of dissemination of S. epidermidis strains resistant to a wide range of clinically important antibiotics.
本研究旨在分析从未使用糖肽类抗生素治疗过的患者身上分离出的三株耐替考拉宁表皮葡萄球菌的 DNA 序列。
采用肉汤微量稀释法测定 12 种抗生素（包括替考拉宁和万古霉素）的最小抑菌浓度（MIC）。提取基因组 DNA，采用 HiSeqX 配对末端测序，并使用 MyPro 管道将其组装成草图基因组序列。
对草图基因组序列的分析表明，耐替考拉宁的表皮葡萄球菌分离株属于多位点序列分型（MLST）序列型 ST5 和 ST87，并编码多种抗菌药物耐药基因，包括耐甲氧西林基因 mecA。
本报告强调了对临床重要抗生素具有广泛耐药性的表皮葡萄球菌菌株传播的风险。
