Research Center of Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, Beijing, 100012, PR China; College of Water Sciences, Beijing Normal University, Beijing, 100875, PR China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environment Sciences, Beijing, 100012, PR China.
Research Center of Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, Beijing, 100012, PR China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environment Sciences, Beijing, 100012, PR China.
J Hazard Mater. 2019 May 5;369:465-473. doi: 10.1016/j.jhazmat.2019.02.034. Epub 2019 Feb 15.
The performance of the microaeration hydrolytic acidification (MAHA) process and microbial community were investigated under different organic loading rates (OLRs) for the pretreatment of 2-butenal manufacture wastewater (2-BMW). Results indicated that OLRs had different impact on the performance of MAHA process. More than 23.7 ± 2.3% of the chemical oxygen demand (COD) removal and the highest acidification degree (20.9 ± 3.1%) were obtained when OLRs were less than 4.0 ± 0.1 kgCOD/m d. However, further increasing OLRs to 6.1 ± 0.1 kgCOD/m d subsequently led to the significant reductions of COD removal and acidification degree. In addition, it could be preliminarily inferred that 2H-pyran-2-one tetrahydro-4-(2-methyl-1-propen-3-yl), 5-formyl-6-methyl-4,5-dihydropyran and ethyl sorbate were the main refractory and toxic organics for microorganisms in the wastewater. The soluble microbial product (SMP) and extracellular polymeric substance (EPS) contents (protein, polysaccharide, nucleic acid) had obvious changes under different OLRs. With parallel factor (PARAFAC) model, four fluorescent components were identified. The F of protein-like substances in SMP significantly decreased with increasing OLRs to 6.1 ± 0.1 kgCOD/m d, which might attribute to fluorescence quenching. Illumina MiSeq sequencing revealed that Pseudomonas, Longilinea, T78, Clostridium, WCHB1-05, Acinetobacter, SHD-231 and Oscillospira were dominant genera at different OLRs.
该研究考察了不同有机负荷率（OLR）下微曝气水解酸化（MAHA）工艺的性能和微生物群落，以预处理 2-丁烯醛生产废水（2-BMW）。结果表明，OLR 对 MAHA 工艺的性能有不同的影响。当 OLR 小于 4.0 ± 0.1 kgCOD/m·d 时，COD 去除率超过 23.7 ± 2.3%，酸化程度最高（20.9 ± 3.1%）。然而，进一步将 OLR 提高到 6.1 ± 0.1 kgCOD/m·d 会导致 COD 去除率和酸化程度的显著降低。此外，可以初步推断，2H-吡喃-2-酮四氢-4-（2-甲基-1-丙烯-3-基）、5-甲酰基-6-甲基-4,5-二氢吡喃和乙基山梨酸酯是废水中微生物的主要难降解和有毒有机物。不同 OLR 下可溶性微生物产物（SMP）和胞外聚合物（EPS）含量（蛋白质、多糖、核酸）有明显变化。采用平行因子（PARAFAC）模型，鉴定出四个荧光组分。SMP 中蛋白样物质的 F 值随着 OLR 增加到 6.1 ± 0.1 kgCOD/m·d 而显著降低，这可能归因于荧光猝灭。Illumina MiSeq 测序结果表明，在不同 OLR 下，假单胞菌、长形菌、T78、梭菌、WCHB1-05、不动杆菌、SHD-231 和 Oscillospira 是优势属。
