Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.
Food Chem. 2019 Jul 1;285:111-118. doi: 10.1016/j.foodchem.2019.01.127. Epub 2019 Jan 31.
Probiotic lactic acid bacteria (LAB) are generally employed in food industry because they contribute to nutritional value of fermented foods. Although knowledge of LAB composition is of high relevance for various industrial and biotechnological applications, the comprehensive identification of LAB species is sometimes technically challenging. Recently, MALDI-TOF MS-based methodologies for bacteria detection/identification in clinical diagnostics and agri-food proved to be an attractive strategy, complementary to traditional techniques for their sensitivity and specificity. In this study, we propose, for the first time, a novel methodology based on high resolution nano-LC-ESI-MS/MS for LAB identification at genus, species and sub-species level by using the sequence regions 33-52 and 72-82 of the S16 ribosomal protein as proteotypic peptide markers. The developed methodology was then applied to the analyses of buffalo and bovine whey starter cultures, thus assessing the applicability of the approach for the detection of LAB also in complex matrices.
益生菌乳酸菌（LAB）通常被应用于食品工业，因为它们有助于发酵食品的营养价值。尽管 LAB 组成的知识对于各种工业和生物技术应用具有高度相关性，但 LAB 物种的全面鉴定有时在技术上具有挑战性。最近，MALDI-TOF MS 基方法在临床诊断和农业食品中的细菌检测/鉴定方面被证明是一种有吸引力的策略，与传统技术相比，它们具有灵敏度和特异性。在这项研究中，我们首次提出了一种基于高分辨 nano-LC-ESI-MS/MS 的新方法，通过使用 S16 核糖体蛋白的序列区域 33-52 和 72-82 作为特征肽标记物，在属、种和亚种水平上对 LAB 进行鉴定。然后将所开发的方法应用于水牛和牛乳清 starter 培养物的分析，从而评估该方法在复杂基质中检测 LAB 的适用性。
