百里香酚和水杨酸对根霉细胞膜和线粒体的抗真菌作用及其在番茄采后保鲜中的应用。
Antifungal effects of thymol and salicylic acid on cell membrane and mitochondria of Rhizopus stolonifer and their application in postharvest preservation of tomatoes.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; International Joint Laboratory on Food Safety, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; International Joint Laboratory on Food Safety, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China.
出版信息
Food Chem. 2019 Jul 1;285:380-388. doi: 10.1016/j.foodchem.2019.01.099. Epub 2019 Jan 23.
This study investigated effects of the simultaneous application of thymol and salicylic acid (SIM) on the target sites of Rhizopus stolonifer, as well as the defenceenzymes of postharvest tomato, when applied as edible coating. SIM induced the changes of ultrastructure and membrane integrity of R. stolonifer. When the concentrations of the fungistat increased, cells stained with propidium iodide and leakage of 260/280 nm-absorbing materials increased while ergosterol synthesis decreased, suggesting damage of cell membrane. Furthermore, SIM treatment significantly reduced the citric acid content and the activities of enzymes related to the tricarboxylic acid cycle, and increased the mitochondrial membrane potential and the reactive oxygen species, indicating damage of mitochondrial-related functions. Moreover, SIM edible coating increased the defence enzyme activities in tomato. Based on the results, SIM can be used as a potential preservation method for tomato as it showed a targeted effect on the cell membrane and mitochondria of R. stolonifer.
本研究探讨了百里香酚和水杨酸（SIM）同时应用于作为可食用涂层时对根霉（Rhizopus stolonifer）靶位的影响，以及对采后番茄防御酶的影响。SIM 诱导根霉的超微结构和膜完整性发生变化。当抑菌剂浓度增加时，碘化丙啶染色的细胞和 260/280nm 吸光度物质的泄漏增加，而麦角固醇合成减少，表明细胞膜受损。此外，SIM 处理显著降低了柠檬酸含量和与三羧酸循环相关的酶的活性，增加了线粒体膜电位和活性氧，表明与线粒体相关的功能受到损害。此外，SIM 可食用涂层增加了番茄中防御酶的活性。基于这些结果，SIM 可用作番茄的潜在保鲜方法，因为它对根霉的细胞膜和线粒体表现出靶向作用。
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