College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
Teagasc Food Research Centre, Moorepark, Fermoy, Co.Cork, Ireland.
Appl Microbiol Biotechnol. 2019 Sep;103(18):7663-7674. doi: 10.1007/s00253-019-09991-w. Epub 2019 Jul 11.
The inhibitory effect of Bacillomycin D, a cyclic lipopeptide, on Rhizopus stolonifer colonization of cherry tomato was studied, and its possible mechanism of action was explored. Bacillomycin D showed a direct inhibitory effect on R. stolonifer spore germination and mycelial growth in vitro. It conferred both a direct inhibitory effect on R. stolonifer growth in cherry tomato in vivo and induced host resistance in cherry tomato. Moreover, Bacillomycin D treatment significantly increased the activities of plant defense-related enzymes, including chitinase (CHI), β-1,3-glucanase (GLU), phenylalanine ammonia-lyase (PAL), and peroxidase (POD). Real-time PCR (RT-PCR) showed that defense-related genes involved in the salicylic acid defense signaling pathway and genes encoding pathogenesis-related proteins were up-regulated in Bacillomycin D treatment. Furthermore, Bacillomycin D-C16 resulted in direct inhibition and a remarkable induced resistance to R. stolonifer which was higher than as induced by Bacillomycin D-C14. Together, the data indicated that Bacillomycin D can control the growth of R. stolonifer through both the direct inhibition of the fungus and the activation of defense-related genes and enzymes in cherry tomato.
研究了环状脂肽 Bacillomycin D 对樱桃番茄根霉属（Rhizopus stolonifer）定殖的抑制作用，并探讨了其可能的作用机制。 Bacillomycin D 在体外对根霉属孢子萌发和菌丝生长具有直接抑制作用。它对樱桃番茄体内根霉属的生长具有直接抑制作用，并诱导樱桃番茄产生宿主抗性。此外， Bacillomycin D 处理显著增加了植物防御相关酶的活性，包括几丁质酶（CHI）、β-1,3-葡聚糖酶（GLU）、苯丙氨酸解氨酶（PAL）和过氧化物酶（POD）。实时 PCR（RT-PCR）显示，参与水杨酸防御信号通路的防御相关基因和编码病程相关蛋白的基因在 Bacillomycin D 处理下上调。此外， Bacillomycin D-C16 对根霉属的直接抑制作用和诱导抗性均显著高于 Bacillomycin D-C14， Bacillomycin D-C16 是 Bacillomycin D 的类似物。综上所述，数据表明 Bacillomycin D 可以通过直接抑制真菌和激活樱桃番茄中防御相关基因和酶来控制根霉属的生长。
