College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China; College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China.
School of Agriculture and Food Sciences, University of Queensland, QLD 4046, Australia.
Food Chem. 2019 Jul 1;285:441-449. doi: 10.1016/j.foodchem.2019.01.120. Epub 2019 Jan 24.
Malolactic fermentation (MLF), usually induced by Oenococcus oeni (O. oeni), is an important process to improve wine quality. Acid acclimation has been proven to be useful for enhancing the viability of lyophilized O. oeni. To explain the involved mechanisms, cell integrity, morphology and protein patterns of lyophilized O. oeni SD-2a were investigated with acid acclimation. After lyophilization, improvement of cell integrity and more extracellular polymeric substances (EPS) were observed in acid acclimated cells. Combined with GO and KEGG analysis, different abundant proteins were noticeably enriched in the carbohydrate metabolism process, especially amino sugar and nucleotide sugar metabolism. The most significant result was the over-expression of proteins participating in cell wall biosynthesis, EPS production, ATP binding and the bacterial secretion system. This result indicated the important role of acid acclimation on cell envelope properties. In addition, protein response to stress and arginine deiminase pathway were also proven to be over-expressed.
苹果酸-乳酸发酵（MLF）通常由片球菌属（Oenococcus oeni）诱导，是提高葡萄酒质量的重要过程。已证明酸驯化有助于提高冻干片球菌属（O. oeni）的存活率。为了解释所涉及的机制，对经过酸驯化的冻干 O. oeni SD-2a 的细胞完整性、形态和蛋白质模式进行了研究。经过冻干后，在经过酸驯化的细胞中观察到细胞完整性的改善和更多的胞外多聚物（EPS）。结合 GO 和 KEGG 分析，在碳水化合物代谢过程中，特别是氨基糖和核苷酸糖代谢中，明显富集了不同丰度的蛋白质。最显著的结果是参与细胞壁生物合成、EPS 产生、ATP 结合和细菌分泌系统的蛋白质表达增加。这一结果表明酸驯化对细胞膜特性的重要作用。此外，还证明了应激蛋白反应和精氨酸脱氨酶途径的过度表达。
