Hoffer L J, Beitins I Z, Kyung N H, Bistrian B R
J Clin Endocrinol Metab. 1986 Feb;62(2):288-92. doi: 10.1210/jcem-62-2-288.
The reproductive hormone response to severe caloric restriction (600 Cal day-1) was studied in six men 33-67% over ideal body weight who completed a 32-day protocol consisting of three periods in the following order: control (4 days), maintenance protein and energy; diet A (14 days), 50 g lean beef protein plus 50 g casein; and diet B (14 days), 50 g lean beef protein plus 50 g carbohydrate. Weight loss (8.7-12.5 kg) was associated with a decrease in mean blood glucose [4.52 +/- 0.60 (+/- SEM), 3.49 +/- 0.29, and 3.80 +/- 0.30 mM] and an increase in beta-hydroxybutyrate (less than 0.10, 2.09 +/- 0.44, and 1.06 +/- 0.34 mM), as determined on the final morning of each period. On the same days, mean serum FSH and LH responses to LHRH infusion of 0.2 micrograms min-1 for 4 h (expressed as milliinternational units per ml area under the concentration-time curve) were: FSH, 1558 +/- 359, 1336 +/- 545, and 1337 +/- 321 (P = NS); and LH, 1730 +/- 545, 1612 +/- 481, and 1782 +/- 556 (P = NS), respectively. Basal serum FSH, LH, free testosterone (T), and total T changed, while 24-h urinary LH and FSH excretion increased on diet A only. Unlike 10 days of total fasting, during which the same amount of weight was lost, basal serum FSH and LHRH-stimulated serum FSH responses were both significantly diminished by 25%, and serum T was diminished by 19% (1), these same parameters were little changed by either low energy diet. The increased urinary excretion of FSH and LH during diet A suggests that greater ketosis increases renal gonadotropin clearance.
对6名体重超过理想体重33 - 67%的男性进行了研究，观察他们在严格限制热量摄入（每天600千卡）情况下的生殖激素反应。这些男性完成了一个为期32天的实验方案，该方案按以下顺序分为三个阶段：对照期（4天），摄入维持蛋白质和能量的饮食；饮食A期（14天），摄入50克瘦牛肉蛋白加50克酪蛋白；饮食B期（14天），摄入50克瘦牛肉蛋白加50克碳水化合物。体重减轻（8.7 - 12.5千克）与平均血糖降低[4.52±0.60（±标准误）、3.49±0.29和3.80±0.30毫摩尔]以及β-羟丁酸增加（低于0.10、2.09±0.44和1.06±0.34毫摩尔）相关，这些数据是在每个阶段的最后一个早晨测定的。在同一天，平均血清促卵泡生成素（FSH）和促黄体生成素（LH）对以每分钟0.2微克的速度输注促性腺激素释放激素（LHRH）4小时的反应（以每毫升浓度 - 时间曲线下面积的毫国际单位表示）分别为：FSH，1558±359、1336±545和1337±321（P = 无显著差异）；LH，1730±545、1612±481和1782±556（P = 无显著差异）。基础血清FSH、LH、游离睾酮（T）和总睾酮发生了变化，而仅在饮食A期24小时尿LH和FSH排泄量增加。与10天完全禁食期间体重减轻量相同但基础血清FSH和LHRH刺激的血清FSH反应均显著降低25%且血清T降低19%（1）不同，这两种低能量饮食对这些相同参数几乎没有影响。饮食A期FSH和LH尿排泄量增加表明酮症程度增加会提高肾脏促性腺激素清除率。
