评估来自泰国和台湾地区近期过期及陈旧抗蛇毒血清产品的理化性质和功效。
Evaluating the physicochemical properties and efficacy of recently expired and aged antivenom products from Thailand and Taiwan.
作者信息
Tan Kae Yi, Liew Sin Teng, Tan Qian Yi, Abdul-Rahman Farah Nadhirah, Azmi Nor Izzati, Sim Si Mui, Tan Nget Hong, Khomvilai Sumana, Sitprija Visith, Tan Choo Hock
机构信息
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Department of Biomedical Sciences, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
出版信息
Toxicon. 2019 Mar 15;160:55-58. doi: 10.1016/j.toxicon.2019.02.010. Epub 2019 Feb 22.
Gel filtration chromatography and gel electrophoresis revealed minimal protein degradation in lyophilized antivenoms which were 2-year expired (Hemato Polyvalent, Neuro Polyvalent; Thailand) and 18-year expired (Hemato Bivalent, Neuro Bivalent; Taiwan). All expired antivenoms retained immunological binding activity, and were able to neutralize the hemotoxic or neurotoxic as well as lethal effects of the homologous snake venoms. The findings show that antivenoms under proper storage conditions may remain relatively stable beyond the indicated shelf life.
凝胶过滤色谱法和凝胶电泳显示，过期2年的冻干抗蛇毒血清（泰国的血液多价、神经多价抗蛇毒血清）和过期18年的冻干抗蛇毒血清（台湾的血液二价、神经二价抗蛇毒血清）中蛋白质降解极少。所有过期抗蛇毒血清均保留免疫结合活性，并且能够中和同源蛇毒的血液毒性或神经毒性以及致死作用。研究结果表明，在适当储存条件下，抗蛇毒血清在标明的保质期之后可能仍相对稳定。
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