Department of Molecular Genetics, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA.
Department of Molecular Genetics, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA.
Curr Opin Cell Biol. 2019 Jun;58:42-49. doi: 10.1016/j.ceb.2019.01.002. Epub 2019 Feb 22.
Eukaryotic cells rely on flux of macromolecules between the nucleus and the cytoplasm for growth and survival. Bidirectional transport is achieved through Nuclear Pore Complexes (NPCs) embedded in the Nuclear Envelope (NE). NPC proteins perform other cellular functions during mitosis, chromatin organization, DNA repair and gene regulation. Dysregulation of NPC number, or defects in their structure and function, are linked to numerous diseases but how NPCs are faithfully inherited during mitosis is poorly understood. In this review, we discuss recent insights to mechanisms of mammalian mitotic NPC segregation and NPC assembly as well as mitotic NPC inheritance via the mitotic chromatin located NPC protein Nup2 in Aspergillus nidulans. We suggest mitotic Nup2 chromatin-based mechanisms could also operate in vertebrate cells.
真核细胞依赖于核质之间大分子的流动来实现生长和存活。通过嵌入核膜（NE）中的核孔复合物（NPC）实现双向运输。在有丝分裂、染色质组织、DNA 修复和基因调控过程中，NPC 蛋白执行其他细胞功能。NPC 数量的失调，或其结构和功能的缺陷，与许多疾病有关，但 NPC 如何在有丝分裂过程中被准确地遗传尚不清楚。在这篇综述中，我们讨论了最近关于哺乳动物有丝分裂 NPC 分离和 NPC 组装的机制的见解，以及通过位于有丝分裂核小体的 NPC 蛋白 Nup2 在构巢曲霉中的有丝分裂 NPC 遗传。我们认为有丝分裂 Nup2 基于染色质的机制也可能在脊椎动物细胞中起作用。
