Department of Life Science and Biochemical Engineering, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam, 31460, Republic of Korea.
Department of Pharmaceutical Engineering and Biotechnology, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam, 31460, Republic of Korea.
Appl Microbiol Biotechnol. 2019 Apr;103(7):2959-2972. doi: 10.1007/s00253-019-09672-8. Epub 2019 Feb 23.
Resveratrol (3,5,4'-trihydroxystilbene) and piceatannol (3,5,3',4'-tetrahydroxystilbene) are well-known natural products that are produced by plants. They are important ingredients in pharmaceutical industries and nutritional supplements. They display a wide spectrum of biological activity. Thus, the needs for these compounds are increasing. The natural products have been found in diverse plants, mostly such as grapes, passion fruit, white tea, berries, and many more. The extraction of these products from plants is quite impractical because of the low production in plants, downstream processing difficulties, chemical hazards, and environmental issues. Thus, alternative production in microbial hosts has been devised with combinatorial biosynthetic systems, including metabolic engineering, synthetic biology, and optimization in production process. Since the biosynthesis is not native in microbial hosts such as Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum, genetic engineering and manipulation have made it possible. In this review, the discussion will mainly focus on recent progress in production of resveratrol and piceatannol, including the various strategies used for their production.
白藜芦醇（3,5,4'-三羟基二苯乙烯）和皮考汀（3,5,3',4'-四羟基二苯乙烯）是两种众所周知的天然产物，它们由植物产生。它们是制药行业和营养补充剂的重要成分。它们具有广泛的生物活性。因此，对这些化合物的需求正在增加。这些天然产物在许多植物中都有发现，主要有葡萄、西番莲、白茶、浆果等。由于植物中产量低、下游加工困难、化学危害和环境问题，从植物中提取这些产品是不切实际的。因此，人们设计了在微生物宿主中替代生产的方法，包括组合生物合成系统，包括代谢工程、合成生物学和生产过程的优化。由于生物合成在微生物宿主如大肠杆菌、酿酒酵母和谷氨酸棒杆菌中不是天然的，因此遗传工程和操作使其成为可能。在这篇综述中，讨论将主要集中在白藜芦醇和皮考汀生产的最新进展上，包括用于生产它们的各种策略。
