通过数字聚合酶链反应分析主要 BCR-ABL1 mRNA 有助于预测国际标准。
Analysis of major BCR-ABL1 mRNA by digital polymerase chain reaction is useful for prediction of international scale.
机构信息
Division of Laboratory Medicine, Sapporo Medical University Hospital, South 1, West 16, Chuo-ku, Sapporo, 060-8543, Japan.
Department of Infection Control and Laboratory Medicine, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, Sapporo, 060-8543, Japan.
出版信息
Int J Clin Oncol. 2019 Jul;24(7):871-875. doi: 10.1007/s10147-019-01419-9. Epub 2019 Feb 23.
BACKGROUND
Major BCR-ABL1 mRNA in patients with chronic myeloid leukemia (CML) has generally been analysed by real-time polymerase chain reaction (PCR). Application of the international scale (IS) for the quantification of major BCR-ABL1 mRNA has been recommended in several sets of guidelines, including those of the European LeukemiaNet. The aim of this study was to clarify the efficacy of digital PCR technology for the IS of BCR-ABL1 mRNA in the patients with CML by comparing with real-time PCR.
METHODS
The analysis of BCR-ABL1 mRNA was carried out by the Ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit (Qiagen), and the QuantStudio 3D Digital PCR System (Thermo Fisher Scientific) using 20 peripheral blood samples obtained from the 9 patients with CML at Sapporo Medical University Hospital.
RESULTS
The correlation between the data obtained by digital PCR and by real-time PCR was really high at R = 0.96. The detection limit of digital PCR was up to 0.003% and was equal to IS with 0.01% or less in comparison with real-time PCR.
CONCLUSIONS
Digital PCR technology is promising for predicting the IS value with similar efficacy to real-time PCR and should be useful for simple monitoring of the effects of tyrosine kinase inhibitor (TKI) treatments.
背景
慢性髓性白血病（CML）患者的主要 BCR-ABL1 mRNA 通常通过实时聚合酶链反应（PCR）进行分析。国际标准化（IS）已被推荐用于定量主要 BCR-ABL1 mRNA，包括欧洲白血病网络的指南。本研究旨在通过与实时 PCR 比较，阐明数字 PCR 技术在 CML 患者中 IS 分析 BCR-ABL1 mRNA 的效果。
方法
使用 Ipsogen BCR-ABL1 Mbcr IS-MMR DX 试剂盒（Qiagen）和 Thermo Fisher Scientific 的 QuantStudio 3D 数字 PCR 系统分析 BCR-ABL1 mRNA，该系统使用来自札幌医科大学医院的 9 名 CML 患者的 20 份外周血样本。
结果
数字 PCR 获得的数据与实时 PCR 之间的相关性非常高，R = 0.96。数字 PCR 的检测限高达 0.003%，与实时 PCR 相比，其 IS 为 0.01%或更低。
结论
数字 PCR 技术在预测 IS 值方面具有与实时 PCR 相当的功效，有望用于简单监测酪氨酸激酶抑制剂（TKI）治疗效果。
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