Kesler K A, Herring M B, Arnold M P, Glover J L, Park H M, Helmus M N, Bendick P J
J Vasc Surg. 1986 Jan;3(1):58-64. doi: 10.1067/mva.1986.avs0030058.
Successful development of a vascular prosthesis lined with endothelium may depend on the ability of the attached cells to resist shear forces after implantation. The purpose of this article is to describe a model for measurement of endothelial detachment caused by shear stress and to identify biomaterials that resist loss of attached cells as a result of shear stress. With human umbilical venous endothelium labeled with indium 111-oxine, cellular attachment to uncoated and fibronectin-coated polyester elastomer and expanded polytetrafluoroethylene (e-PTFE) graft surfaces was quantified after an 18-hour incubation. PTFE grafts prepared by immediate seeding were also studied. The relative strength of endothelial attachment was determined by the percentage of the original inoculum remaining after the seeded graft surfaces were subjected to a physiologic shear stress of 15 dynes/cm2 during in vitro perfusion. In polyester elastomer grafts, fibronectin did not significantly increase initial attachment but did increase the percentage of inoculum remaining after perfusion (92.1% vs. 39.74%, p = 0.001). A similar relationship existed between fibronectin-coated e-PTFE and immediately seeded e-PTFE preparations with 61.6% and 25.8%, respectively, of the inoculum remaining after perfusion (p = 0.001). Furthermore, the percentage of inoculum retained on fibronectin-coated polyester elastomer was significantly greater than on fibronectin-coated e-PTFE (p = 0.001). In comparing uncoated grafts, polyester elastomer had 39.7% of the inoculum retained after perfusion whereas only 1.8% was remaining on the e-PTFE grafts (p = 0.0001). We conclude that polyester elastomer permits better endothelial cell attachment than e-PTFE and that fibronectin coating enhances the strength of attachment to both graft materials.
成功开发内衬内皮细胞的血管假体可能取决于植入后附着细胞抵抗剪切力的能力。本文的目的是描述一种用于测量由剪切应力引起的内皮细胞脱离的模型，并确定能够抵抗因剪切应力导致的附着细胞损失的生物材料。用铟 - 111 - 奥辛标记人脐静脉内皮细胞，在孵育18小时后，对未涂层和纤连蛋白涂层的聚酯弹性体以及膨体聚四氟乙烯（e - PTFE）移植物表面的细胞附着情况进行定量分析。还研究了通过即时接种制备的PTFE移植物。通过在体外灌注过程中，将接种后的移植物表面施加15达因/平方厘米的生理剪切应力后，剩余原始接种物的百分比来确定内皮细胞附着的相对强度。在聚酯弹性体移植物中，纤连蛋白并未显著增加初始附着，但确实增加了灌注后剩余接种物的百分比（92.1%对39.74%，p = 0.001）。纤连蛋白涂层的e - PTFE与即时接种的e - PTFE制剂之间存在类似关系，灌注后分别有61.6%和25.8%的接种物留存（p = 0.001）。此外，纤连蛋白涂层的聚酯弹性体上留存的接种物百分比显著高于纤连蛋白涂层的e - PTFE（p = 0.001）。在比较未涂层的移植物时，聚酯弹性体在灌注后有39.7%的接种物留存，而e - PTFE移植物上仅留存1.8%（p = 0.0001）。我们得出结论，聚酯弹性体比e - PTFE更能促进内皮细胞附着，并且纤连蛋白涂层增强了对两种移植物材料的附着强度。
