Seeger J M, Klingman N
J Surg Res. 1985 Jun;38(6):641-7. doi: 10.1016/0022-4804(85)90087-3.
A possible approach to the low seeding efficiency of endothelial cells into prosthetic grafts is to increase the number of cells to be seeded in cell culture and improve seeding efficiency by graft precoating with fibronectin. The effect of cell culture on cell adhesion is unknown, however, and fibronectin also binds fibrin, which may increase the thrombogenicity of the graft luminal surface. To investigate these questions, freshly harvested canine jugular vein endothelial cells from six animals and similar cells harvested from six primary and eight secondary cell cultures were labeled with 111Indium and seeded into 5 cm, 4 mm PTFE grafts coated with fibronectin, using similar uncoated PTFE grafts as controls. Platelet accumulation and distribution on six similar coated and uncoated grafts placed in canine carotid, external jugular arterial venous shunts for 2 hr were also determined using autogenous 111Indium-labeled platelets. Significant differences between group means were determined using the paired Student's t test. Results reveal that seeding efficiency is significantly better in all groups of coated grafts compared to uncoated grafts (P less than 0.01). Cells derived from cell culture also had significantly higher seeding efficiencies than freshly harvested cells when seeded into coated grafts (P less than 0.05) and tended to have higher seeding efficiencies than harvested cells when seeded into uncoated grafts (P = 0.53). Fibronectin coating increased mean platelet accumulation on the entire graft luminal surface, but not to a statistically significant degree (P greater than 0.1). Whether this increased seeding efficiency will improve graft endothelialization remains to be investigated.
提高内皮细胞接种到人工血管移植物中接种效率的一种可能方法是增加细胞培养中接种的细胞数量，并通过用纤连蛋白预涂覆移植物来提高接种效率。然而，细胞培养对细胞黏附的影响尚不清楚，并且纤连蛋白也会结合纤维蛋白，这可能会增加移植物管腔表面的血栓形成性。为了研究这些问题，将来自6只动物的新鲜采集的犬颈静脉内皮细胞以及从6个原代和8个传代细胞培养物中收获的类似细胞用铟 - 111标记，并接种到涂有纤连蛋白的5厘米、4毫米聚四氟乙烯移植物中，使用类似的未涂覆聚四氟乙烯移植物作为对照。还使用自体铟 - 111标记的血小板测定了放置在犬颈动脉、颈外动静脉分流中2小时的6个类似涂覆和未涂覆移植物上的血小板聚集和分布情况。使用配对学生t检验确定组均值之间的显著差异。结果显示，与未涂覆的移植物相比，所有涂覆移植物组的接种效率均显著更高（P小于0.01）。当接种到涂覆移植物中时，源自细胞培养的细胞的接种效率也显著高于新鲜采集的细胞（P小于0.05），并且当接种到未涂覆移植物中时，其接种效率往往高于采集的细胞（P = 0.53）。纤连蛋白涂层增加了整个移植物管腔表面的平均血小板聚集，但未达到统计学显著程度（P大于0.1）。这种提高的接种效率是否会改善移植物内皮化仍有待研究。
