McGee Charles E, Sample Christopher J, Kilburg-Basnyat Brita, Gabor Kristin A, Fessler Michael B, Gowdy Kymberly M
Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC, USA.
Department of Pharmacology and Toxicology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
Methods Mol Biol. 2019;1960:191-205. doi: 10.1007/978-1-4939-9167-9_17.
Laboratory rodent influenza infection models have been and continue to be a critical tool for understanding virus-host interactions during infection. The incidence of seasonal influenza infections coupled with the need for novel therapeutics and universal vaccines highlights the need to uncover novel mechanisms of pathogenesis and protection. Mouse models are extremely useful for the evaluation of influenza vaccines and provide an invaluable tool to probe the immune response. This chapter describes the technique of intranasal inoculation of male C57BL/6J mice with an H1N1 strain of influenza (A/Puerto Rico/8/1934) and methods for assessing the optimum dose for infection, viral titers in lung tissue, and severity of disease.
实验用啮齿动物流感感染模型一直以来都是且仍将是理解感染过程中病毒与宿主相互作用的关键工具。季节性流感感染的发生率，再加上对新型疗法和通用疫苗的需求，凸显了揭示发病机制和保护新机制的必要性。小鼠模型对于评估流感疫苗极为有用，并为探究免疫反应提供了宝贵工具。本章描述了用甲型流感病毒H1N1毒株（A/波多黎各/8/1934）对雄性C57BL/6J小鼠进行鼻内接种的技术，以及评估感染最佳剂量、肺组织病毒滴度和疾病严重程度的方法。
