Gong Wei, Wen Haitao
Department of Hepatobiliary Surgery and Liver Transplantation, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Department of Microbial Infection and Immunity, The Ohio State University College of Medicine, Columbus, OH, USA.
Methods Mol Biol. 2019;1960:249-255. doi: 10.1007/978-1-4939-9167-9_22.
Despite advances in intensive care unit interventions, including the use of specific antibiotics and anti-inflammation treatment, sepsis with concomitant multiple organ failure is the most common cause of death in many acute care units. In order to understand the mechanisms of clinical sepsis and develop effective therapeutic modalities, there is a need to use effective experimental models that faithfully replicate what occurs in patients with sepsis. Several models are commonly used to study sepsis, including intravenous endotoxin challenge, injection of live organisms into the peritoneal cavity, establishing abscesses in the extremities, and the induction of experimental polymicrobial peritonitis via cecal ligation and puncture (CLP). Here, we describe the surgical procedure of CLP in mice, which has been demonstrated to closely replicate the nature and course of clinical sepsis in human subjects.
尽管重症监护病房的干预措施取得了进展，包括使用特定的抗生素和抗炎治疗，但伴有多器官功能衰竭的脓毒症仍是许多急性护理病房中最常见的死亡原因。为了了解临床脓毒症的机制并开发有效的治疗方法，需要使用能够忠实地复制脓毒症患者所发生情况的有效实验模型。几种模型通常用于研究脓毒症，包括静脉内注射内毒素、将活生物体注入腹腔、在四肢形成脓肿以及通过盲肠结扎和穿刺（CLP）诱导实验性多微生物腹膜炎。在此，我们描述了小鼠CLP的手术过程，该过程已被证明能密切复制人类临床脓毒症的性质和病程。
