Sinclair Erik, Magnusson Maria K, Angenete Eva, Prytz Mattias, Tasselius Viktor, Öhman Lena, Haglind Eva
Department of Surgery, Scandinavian Surgical Outcomes Research Group, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Surgery, Region Västra Götaland, NU-Hospital Group, Trollhättan, Sweden.
Eur J Med Res. 2025 Mar 18;30(1):180. doi: 10.1186/s40001-025-02445-2.
Laparoscopic lavage is an effective, safe, and feasible treatment in patients with perforated diverticulitis with purulent peritonitis. Laparoscopic lavage was introduced without any detailed knowledge regarding the mechanisms of action. The aim of this study was to validate the reproducibility of an animal model of purulent peritonitis and to study the effect of laparoscopic lavage on inflammatory proteins in this model.
Forty rats, divided into eight groups (n = 5) were operated. Six groups underwent cecal ligation and puncture (CLP) causing peritonitis and two groups underwent sham surgery. Three CLP and one sham group received laparoscopic lavage, while the remaining groups acted as time-matched controls. Samples of abdominal fluid and blood were collected after 1, 2 or 3 h and analyzed regarding 92 inflammatory proteins using Olink Target 96 Mouse exploratory panel.
Animals with peritonitis had higher levels of inflammatory proteins such as CCL3, IL17A and IL6 in abdominal fluid and serum compared to sham. The groups treated with laparoscopic lavage had lower levels of inflammatory proteins in both abdominal fluid and serum compared with untreated peritonitis groups, results were most distinct sampled after one hour.
Our animal model is reproducible, and mimics perforated diverticulitis with purulent peritonitis with increased levels of inflammatory proteins in abdominal fluid and serum. The levels of several inflammatory proteins were lower following laparoscopic lavage treatment perhaps indicating the physiological effect of laparoscopic lavage. This model can be used to further explore the mechanisms involved in peritonitis and laparoscopic lavage treatment.
腹腔镜灌洗是治疗穿孔性憩室炎合并脓性腹膜炎患者的一种有效、安全且可行的方法。腹腔镜灌洗在引入时对其作用机制并无详细了解。本研究的目的是验证脓性腹膜炎动物模型的可重复性，并研究腹腔镜灌洗对该模型中炎症蛋白的影响。
将40只大鼠分为八组（每组n = 5）并进行手术。六组接受盲肠结扎和穿刺（CLP）以引发腹膜炎，两组接受假手术。三组CLP组和一组假手术组接受腹腔镜灌洗，其余组作为时间匹配的对照组。在1、2或3小时后收集腹腔液和血液样本，并使用Olink Target 96 Mouse探索性面板分析92种炎症蛋白。
与假手术组相比，患有腹膜炎的动物腹腔液和血清中的炎症蛋白如CCL3、IL17A和IL6水平更高。与未治疗的腹膜炎组相比，接受腹腔镜灌洗治疗的组腹腔液和血清中的炎症蛋白水平较低，在1小时后取样时结果最为明显。
我们的动物模型具有可重复性，模拟了穿孔性憩室炎合并脓性腹膜炎，腹腔液和血清中的炎症蛋白水平升高。腹腔镜灌洗治疗后几种炎症蛋白的水平较低，这可能表明了腹腔镜灌洗的生理作用。该模型可用于进一步探索腹膜炎和腹腔镜灌洗治疗所涉及的机制。
