Anderson Sarah E, Rather Philip N
Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA.
Emory Antibiotic Resistance Center, Emory University, Atlanta, GA, USA.
Methods Mol Biol. 2019;1946:151-157. doi: 10.1007/978-1-4939-9118-1_14.
Many strains of Acinetobacter baumannii, including the highly virulent strain AB5075, undergo a high-frequency switch that results in two cell types that are distinguished by their opaque or translucent colony opacities when viewed by oblique lighting. Opaque (VIR-O) and translucent (AV-T) colonies exhibit multiple phenotypic differences, including virulence. Here we describe how to distinguish between VIR-O and AV-T colony variants and how to generate highly pure stocks of each variant. We also describe methods for measuring opacity switching frequencies of cells grown on agar plates and in liquid cultures.
许多鲍曼不动杆菌菌株，包括高毒力菌株AB5075，会发生高频转换，产生两种细胞类型，通过斜射光观察时，它们的菌落透明度分别为不透明或半透明。不透明（VIR-O）和半透明（AV-T）菌落表现出多种表型差异，包括毒力。在这里，我们描述了如何区分VIR-O和AV-T菌落变体，以及如何生成每种变体的高纯度菌株。我们还描述了测量在琼脂平板和液体培养物中生长的细胞的透明度转换频率的方法。
