检测鲍曼不动杆菌中N-酰基高丝氨酸内酯产生的方法。
Methods for Detecting N-Acyl Homoserine Lactone Production in Acinetobacter baumannii.
作者信息
Paulk Tierney Aimee R, Rather Philip N
机构信息
Department of Microbiology and Immunology, Emory University School of Medicine, Emory University, Atlanta, GA, USA.
Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA.
出版信息
Methods Mol Biol. 2019;1946:253-258. doi: 10.1007/978-1-4939-9118-1_23.
Abstract
Acinetobacter baumannii cell-cell signaling is mediated by production and release of N-acyl homoserine lactones (AHLs), primarily N-(3-hydroxydodecanoyl)-L-homoserine lactone (3-hydroxy-C-HSL). The secretion of this signal can be readily assessed via simple plate-based or thin-layer chromatography-based assays utilizing an Agrobacterium tumefaciens traG-lacZ containing biosensor. In this chapter, we describe methods to assay for secreted N-acyl homoserine lactone production in Acinetobacter baumannii.
摘要
鲍曼不动杆菌的细胞间信号传导由N-酰基高丝氨酸内酯（AHLs）的产生和释放介导，主要是N-（3-羟基十二烷酰基）-L-高丝氨酸内酯（3-羟基-C-HSL）。利用含有根癌农杆菌traG-lacZ的生物传感器，通过基于平板或基于薄层色谱的简单测定法，可以很容易地评估这种信号的分泌。在本章中，我们描述了检测鲍曼不动杆菌中分泌的N-酰基高丝氨酸内酯产生的方法。
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