Paulk Tierney Aimee R, Rather Philip N
Department of Microbiology and Immunology, Emory University School of Medicine, Emory University, Atlanta, GA, USA.
Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA.
Methods Mol Biol. 2019;1946:253-258. doi: 10.1007/978-1-4939-9118-1_23.
Acinetobacter baumannii cell-cell signaling is mediated by production and release of N-acyl homoserine lactones (AHLs), primarily N-(3-hydroxydodecanoyl)-L-homoserine lactone (3-hydroxy-C-HSL). The secretion of this signal can be readily assessed via simple plate-based or thin-layer chromatography-based assays utilizing an Agrobacterium tumefaciens traG-lacZ containing biosensor. In this chapter, we describe methods to assay for secreted N-acyl homoserine lactone production in Acinetobacter baumannii.
鲍曼不动杆菌的细胞间信号传导由N-酰基高丝氨酸内酯（AHLs）的产生和释放介导，主要是N-（3-羟基十二烷酰基）-L-高丝氨酸内酯（3-羟基-C-HSL）。利用含有根癌农杆菌traG-lacZ的生物传感器，通过基于平板或基于薄层色谱的简单测定法，可以很容易地评估这种信号的分泌。在本章中，我们描述了检测鲍曼不动杆菌中分泌的N-酰基高丝氨酸内酯产生的方法。
