Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA.
Antimicrob Agents Chemother. 2013 Apr;57(4):1926-9. doi: 10.1128/AAC.02161-12. Epub 2013 Jan 14.
Streptomycin at subinhibitory concentrations was found to inhibit quorum sensing in Acinetobacter baumannii. Conditioned medium prepared by growth of A. baumannii in the presence of subinhibitory concentrations of streptomycin exhibited reduced activation of two quorum-sensing-regulated genes, abaI, encoding an autoinducer synthase, and A1S_0112. The reduced expression of AbaI resulted in greatly decreased levels of 3-OH-C(12)-HSL as confirmed by direct analysis using thin-layer chromatography. The effect on acyl-homoserine lactone (AHL) signal production was specific to streptomycin, as gentamicin and myomycin had no significant effect at subinhibitory levels.
在亚抑菌浓度下，链霉素被发现可以抑制鲍曼不动杆菌的群体感应。由在亚抑菌浓度的链霉素存在下生长的鲍曼不动杆菌制备的条件培养基显示出两个群体感应调节基因 abaI 和 A1S_0112 的激活减少，该基因编码一种自动诱导物合成酶。AbaI 的表达减少导致 3-OH-C(12)-HSL 的水平大大降低，这一点通过使用薄层层析直接分析得到了证实。这种对酰基高丝氨酸内酯 (AHL) 信号产生的影响是链霉素特有的，因为庆大霉素和米霉素在亚抑菌水平下没有显著影响。
