Department of Animal Science and Institute of Milk Genomics, Chonbuk National University, Jeonju 54896, Republic of Korea; Agency of National Food Cluster, Iksan-si, Jeollabuk-do 54576, Republic of Korea.
R&D, CJ Cheil Jedang Corporation, Seoul 04560, Republic of Korea.
J Dairy Sci. 2019 Apr;102(4):2844-2853. doi: 10.3168/jds.2018-15013. Epub 2019 Feb 22.
Synbiotics, a combination of prebiotics and probiotics, produce synergistic effects to promote gastrointestinal health. Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model. Initially, body weight, food intake, and clinical features were dramatically decreased after treatment with DSS, and the addition of LGG, tagatose, or both ameliorated these effects. In our pyrosequencing analysis of fecal microbiota, DSS treatment increased the abundance of Proteobacteria and decreased that of Firmicutes. When LGG and tagatose were administered as synbiotics, the gut microbiota composition recovered from the dysbiosis caused by DSS treatment. In particular, the abundance of Bacteroides, Lactobacillus, and Akkermansia was significantly associated with probiotic, prebiotic, and synbiotic treatments. Taken together, our results suggest that LGG and tagatose as synbiotics can alleviate colitis, and synbiotics could be applied as dietary supplements in dairy foods such as yogurt and cheese.
共生元是益生菌和益生元的组合，它们产生协同作用，促进胃肠道健康。在此，我们研究了鼠李糖乳杆菌 GG 菌株（一种益生菌菌株）和塔格糖（一种益生元）在葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型中的共生元相互作用。最初，用 DSS 处理后，体重、食物摄入量和临床特征显著下降，而添加 LGG、塔格糖或两者都可改善这些效果。在我们对粪便微生物群的焦磷酸测序分析中，DSS 处理增加了变形菌门的丰度，降低了厚壁菌门的丰度。当 LGG 和塔格糖作为共生元给药时，肠道微生物群组成从 DSS 处理引起的失调中恢复。特别是，拟杆菌、乳杆菌和阿克曼氏菌的丰度与益生菌、益生元和共生元处理显著相关。总之，我们的研究结果表明，LGG 和塔格糖作为共生元可以缓解结肠炎，并且共生元可以作为酸奶和奶酪等乳制品中的膳食补充剂应用。
