Marie-Hardy Laura, Khalifé Marc, Slimani Lofti, Pascal-Moussellard Hugues
Service d'orthopédie et de traumatologie, hôpital de la Pitié-Salpêtrière, 47, boulevard de l'hôpital, 75013 Paris, France.
Service d'orthopédie et de traumatologie, hôpital de la Pitié-Salpêtrière, 47, boulevard de l'hôpital, 75013 Paris, France.
Orthop Traumatol Surg Res. 2019 Apr;105(2):361-367. doi: 10.1016/j.otsr.2018.12.008. Epub 2019 Feb 21.
The zebrafish is widely used in research due in part to its readily manipulable genome. Zebrafish models of spinal deformities including scoliosis were developed recently. However, the methods used to assess the spine in these models vary across studies. The primary objective of this study was to investigate the feasibility and modalities of local and regional spine structure evaluation by micro-CT in the normal zebrafish. The secondary objectives were to assess the feasibility of spinal angle measurements in normal zebrafish subjected to external stresses designed to mimic spinal deformities, to determine normal angle values in the coronal and sagittal planes, and to detail the micro-CT features of the zebrafish spine.
Micro-CT is an effective and reproducible tool for determining orthopaedic parameters to characterise the zebrafish spine.
Two observers conducted preliminary analyses on 15 zebrafish including 12 adults (aged 18 months) and 3 juveniles (aged 12 weeks). For the analyses, 6 of the animals were placed in an artificial position to mimic a scoliosis spinal deformity. Micro-CT (Quantum FX Caliper™) was used with 59μm resolution and a 30-mm field of view. Image processing was with RadiAnt DICOM Viewer™ software.
We defined several assessment planes on the 3D micro-CT reconstructions to measure orthopaedic parameters in the sagittal plane (thoracic and maximal kyphotic curves with their apices, length of the various spinal segments, and sagittal index) and coronal plane (Cobb angles, apices, end-vertebrae, coronal alignment, and side of the convexity). Mean thoracic kyphosis was 20.5°±5.0° in the adults and 8.7° in the juveniles. No curvature was apparent in the coronal plane in the zebrafish left in the neutral position. In the zebrafish with artificially induced curves, micro-CT was effective in determining the Cobb angles and apical vertebrae.
This work defines a standardised micro-CT method for assessing the zebrafish spine. In addition, spinal parameter values that can be considered normal were determined, namely, less than 30° of thoracic kyphosis in the sagittal plane and less than 10° in the coronal plane. Our method was effective in assessing induced spinal deformities on micro-CT reconstructions. We hope it will prove of value in future studies of the zebrafish model.
IV.
斑马鱼因其基因组易于操作而被广泛应用于研究。包括脊柱侧弯在内的脊柱畸形斑马鱼模型最近已被开发出来。然而，这些模型中用于评估脊柱的方法在不同研究中有所不同。本研究的主要目的是探讨在正常斑马鱼中通过显微CT评估局部和区域脊柱结构的可行性及方式。次要目的是评估在受到模拟脊柱畸形的外部应力作用的正常斑马鱼中测量脊柱角度的可行性，确定冠状面和矢状面的正常角度值，并详细描述斑马鱼脊柱的显微CT特征。
显微CT是一种用于确定骨科参数以表征斑马鱼脊柱的有效且可重复的工具。
两名观察者对15条斑马鱼进行了初步分析，其中包括12条成年斑马鱼（18个月龄）和3条幼年斑马鱼（12周龄）。为进行分析，将6条动物置于人工位置以模拟脊柱侧弯脊柱畸形。使用分辨率为59μm、视野为30mm的显微CT（Quantum FX Caliper™）。图像处理使用RadiAnt DICOM Viewer™软件。
我们在三维显微CT重建图像上定义了几个评估平面，以测量矢状面（胸椎和最大后凸曲线及其顶点、各脊柱节段的长度以及矢状指数）和冠状面（Cobb角[注1]、顶点、终椎、冠状位对线以及凸侧）的骨科参数。成年斑马鱼的平均胸椎后凸为20.5°±5.0°，幼年斑马鱼为8.7°。处于中立位置的斑马鱼在冠状面未观察到明显弯曲。在人工诱导出弯曲的斑马鱼中，显微CT能够有效地确定Cobb角和顶椎。
本研究确定了一种用于评估斑马鱼脊柱的标准化显微CT方法。此外，还确定了可被视为正常的脊柱参数值，即在矢状面胸椎后凸小于30°，在冠状面小于10°。我们的方法在评估显微CT重建图像上的诱导性脊柱畸形方面是有效的。我们希望它在未来的斑马鱼模型研究中能证明其价值。
IV级
注1：Cobb角是脊柱侧弯中用于衡量脊柱侧弯程度的一个重要角度指标。
