Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Aachen, Germany,
Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Aachen, Germany.
Caries Res. 2019;53(4):447-456. doi: 10.1159/000496401. Epub 2019 Feb 20.
The incorporation of antibacterial agents into dental restorative materials is a promising strategy for secondary caries prevention. Previously, Carolacton affected Streptococcus mutans biofilm formation on composite materials in vitro. The present study evaluated secondary caries formation adjacent to Carolacton-containing composites and conventional restorative materials using an artificial biofilm model. Standardized cavities were prepared in bovine dentin-enamel samples (n = 175) and restored with various dental materials (Tetric EvoCeram [T], GrandioSo composite without [G] and with Carolacton [GC], Grandio Flow without [F] and with Carolacton [FC], GrandioSo containing sodium fluoride [GNaF], and Ketac Fil [K]). After artificial aging, S. mutans was grown on the samples for 7 days. The investigation of gap sizes and secondary caries formation was performed using confocal laser scanning microscopy and transversal microradiography. Median gap size in enamel was 9.4 µm (interquartile range 7.9-12.7). Compared to all other groups significant differences in gap sizes could be observed for Ketac Fil (p < 0.001; Mann-Whitney test). Only GrandioSo composite containing 30% sodium fluoride and Ketac Fil showed significantly smaller lesion areas in enamel (p < 0.001; Mann-Whitney test) than all other groups which was confirmed by the mineral loss data (p < 0.001; Mann-Whitney test). Based on the present in vitro results, it seems that Carolacton-containing composite in the current formulation within the shown simplified monoculture biofilm model is not able to prevent caries formation compared to fluoride-releasing restorative materials.
将抗菌剂纳入牙科修复材料中是预防继发龋的一种有前途的策略。先前，Carolacton 会影响变形链球菌在复合材料上的生物膜形成。本研究使用人工生物膜模型评估了含有 Carolacton 的复合材料和传统修复材料与继发龋相邻的形成情况。在牛牙釉质-牙本质样本（n = 175）上制备标准腔，并使用各种牙科材料（Tetric EvoCeram [T]、不含 Carolacton 的 GrandioSo 复合材料[G]和含 Carolacton 的复合材料[GC]、不含 Carolacton 的 Grandio Flow 复合材料[F]和含 Carolacton 的复合材料[FC]、含氟化钠的 GrandioSo 复合材料[GNaF]和 Ketac Fil [K]）进行修复。人工老化后，将变形链球菌在样本上培养 7 天。使用共聚焦激光扫描显微镜和横切显微放射摄影术来研究间隙大小和继发龋的形成。釉质中的中位数间隙大小为 9.4 µm（四分位距 7.9-12.7）。与所有其他组相比，Ketac Fil 的间隙大小有显著差异（p < 0.001；Mann-Whitney 检验）。只有含有 30%氟化钠的 GrandioSo 复合材料和 Ketac Fil 在釉质中显示出明显较小的病变面积（p < 0.001；Mann-Whitney 检验），而其他所有组均显示出明显较小的病变面积（p < 0.001；Mann-Whitney 检验），这一结果也得到了矿物质损失数据的证实（p < 0.001；Mann-Whitney 检验）。基于目前的体外结果，在显示的简化单培养生物膜模型中，目前配方中的含 Carolacton 复合材料似乎无法防止龋齿形成，而含氟释放性修复材料则可以。
