Department of Restorative Dentistry, Faculty of Dentistry, Gazi University, Ankara, Emek, 06510, Turkey.
Department of Restorative Dentistry, Faculty of Dentistry, Dokuz Eylul University, İzmir, Turkey.
Clin Oral Investig. 2024 Aug 28;28(9):504. doi: 10.1007/s00784-024-05891-0.
To study the effect of incorporating chitosan and fluoride-loaded chitosan nanoparticles into a glass-ionomer cement (GIC) to prevent secondary caries.
A standard cervical cavity (mesio-distal width 6 mm, cervico-occlusal width 2 mm, and depth 2 mm) was prepared on 30 molars for the following restoration groups: group 1, conventional GIC restoration; group 2, chitosan (10%) modified GIC restoration; group 3, fluoride loaded chitosan nanoparticles (10%) modified GIC restoration. The restored teeth were subjected to 1,500 thermal cycles before undergoing a multi-species cariogenic biofilm challenge. The restored teeth were examined by micro-computed tomography (micro-CT), scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDX). Data were analyzed by the one-way ANOVA, Tukey HDS, Kruskal Wallis, and Dunn's test.
Micro-CT determined outer lesion depths for groups 1-3 were: 614 ± 20 μm, 589 ± 17 μm, and 560 ± 19 μm respectively. Both modifications with chitosan and fluoride-loaded chitosan nanoparticles significantly affected outer lesion depth (p < 0.05). The modification with fluoride-loaded chitosan nanoparticles statistically significantly decreased the outer lesion depth compared to all other groups (p < 0.05). SEM/EDX showed an increase of calcium, phosphorus, and fluoride at the root dentine adjacent to the restoration in groups 2 and 3 (modified GIC). This increase was statistically significantly higher in the group modified with fluorine-loaded nano chitosan particles compared to the other groups (p < 0.05).
Incorporation of 10% chitosan and 10% fluoride-loaded chitosan nanoparticles into GIC restorative material can prevent secondary root caries development. 10% fluoride-loaded chitosan nanoparticles were more effective.
Glass ionomer cement modified with fluoride-loaded chitosan nanoparticles may be a promising restorative material in pediatric and preventive dentistry due to their controlled release properties.
研究将壳聚糖和载氟壳聚糖纳米粒子掺入玻璃离子水门汀（GIC）中以预防继发龋的效果。
在 30 颗磨牙上制备标准颈腔（近远中宽度 6mm，颈缘-颌面宽度 2mm，深度 2mm），用于以下修复组：组 1，常规 GIC 修复；组 2，壳聚糖（10%）改性 GIC 修复；组 3，载氟壳聚糖纳米粒子（10%）改性 GIC 修复。修复后的牙齿经过 1500 次热循环，然后进行多物种致龋生物膜挑战。通过微计算机断层扫描（micro-CT）、扫描电子显微镜结合能谱（SEM/EDX）检查修复后的牙齿。采用单因素方差分析、Tukey HDS、Kruskal Wallis 和 Dunn 检验进行数据分析。
micro-CT 确定组 1-3 的外病变深度分别为：614 ± 20μm、589 ± 17μm 和 560 ± 19μm。壳聚糖和载氟壳聚糖纳米粒子的改性均显著影响外病变深度（p<0.05）。载氟壳聚糖纳米粒子的改性与所有其他组相比，外病变深度统计学显著降低（p<0.05）。SEM/EDX 显示在组 2 和 3（改性 GIC）中，与修复体相邻的根牙本质中钙、磷和氟的含量增加。与其他组相比，载氟纳米壳聚糖颗粒改性组的增加统计学上显著更高（p<0.05）。
将 10%壳聚糖和 10%载氟壳聚糖纳米粒子掺入 GIC 修复材料中可预防继发根龋的发展。10%载氟壳聚糖纳米粒子更有效。
由于载氟壳聚糖纳米粒子具有控释特性，因此用载氟壳聚糖纳米粒子改性的玻璃离子水门汀可能成为儿童和预防牙科领域有前途的修复材料。
