叶片乙醇提取物对佐剂和角叉菜胶处理大鼠的抗炎作用。

Anti-inflammatory Effect of Leaves Ethanolic Extract on Adjuvant and Carragenan Treated Rats.

机构信息

Department of Science, CMJ University Jorabat, Raibhoi, Meghalaya-793101, India.

Vinayaka College of Pharmacy, Kullu (HP), India.

出版信息

Antiinflamm Antiallergy Agents Med Chem. 2020;19(2):103-112. doi: 10.2174/1871523018666190222120127.

BACKGROUND

Woodfordia fructicosa is used traditionally for the treatment of inflammation associated with arthritis.

METHODS

In the present study, the anti-inflammatory activity of W. fructicosa (WFE) leaves ethanolic extract was assessed in Sprague Dawley rats by giving 200 mg/kg dose orally. Inflammation was studied by using carrageenan induced paw edema, Freund's adjuvant (FA) and monosodium iodo acetate (MIA) induced arthritis as animal models. Serum tumor necrosis factor-alpha (TNF-α) was estimated in blood sample of animals treated with FA. The one way ANOVA followed by Bonferroni's test was used for statistical analysis.

RESULTS

WFE significantly decreased (P<0.05, P<0.001) paw thickness in carrageenan induced paw edema and FA induced arthritis. The significant decrease in knee diameter (P<0.001) in MIA induced arthritis as well as inhibitory effect (P<0.001) on elevated TNF- α was observed.

CONCLUSION

These results showed that the WFEexerted an inhibitory effect on TNF-α and carrageenan paw edema which may justify its traditional use in inflammatory conditions. Thus, the study shows that leaves of W. fruticose afford anti-inflammatory activity by preventing the inflammation in different animal models.

背景

辣蓼大丁草被传统用于治疗与关节炎相关的炎症。

方法

在本研究中，辣蓼大丁草（WFE）叶乙醇提取物通过口服给予 200mg/kg 剂量，在 Sprague Dawley 大鼠中评估其抗炎活性。通过使用角叉菜胶诱导的爪肿胀、弗氏完全佐剂（FA）和单碘乙酸盐（MIA）诱导的关节炎作为动物模型来研究炎症。在给予 FA 的动物的血液样本中估计血清肿瘤坏死因子-α（TNF-α）。采用单因素方差分析（One Way ANOVA），随后进行 Bonferroni 检验进行统计分析。