Laboratory of Cardiovascular Research and Integrative Pharmacology, College of Pharmacy, University of Sargodha, Sargodha, Pakistan.
Department of Pharmacology, University of Health Sciences, Lahore, 54600, Pakistan.
Inflammopharmacology. 2019 Aug;27(4):781-797. doi: 10.1007/s10787-018-0543-4. Epub 2018 Nov 16.
Clematis orientalis Linn has long been used as ethnopharmacy for the treatment of arthritis. This study is intended to evaluate the curative efficacy of Clematis orientalis in treating polyarthritis in rats. Aqueous ethanolic extract and fractions (hexane, butanol and aqueous) were administered orally at 200 mg/kg for 28 days after CFA immunization. Paw swelling, paw diameter, arthritic score, body weight, hematological parameters, radiographic and histological analysis of ankle joints were evaluated. Moreover, levels of various inflammatory markers through RT-PCR and ELISA were measured. DPPH and reducing power assays were used to appraise antioxidant capacity. Qualitative phytochemical analysis, determination of total phenolic and flavonoid contents were also carried out. Aqueous ethanolic extract and fractions significantly (p < 0.001) reduced paw volume, paw thickness and arthritic score and considerably prevented decrease in body weight along with anomalous alterations in hematological parameters in comparison with arthritic control. X-ray and histological examination revealed no significant structural changes in ankle joints of treated rats. Expression levels of IL-1β, TNF-α, IL-6, COX-2 and NF-Kβ were significantly (p < 0.05-0.001) suppressed as well as noteworthy increase in the levels of IL-4 and IL-10 among treated animals has been detected. Overproduction of TNF-α and PGE2 was substantially prevented in animals given different treatments. Aqueous ethanol extract and its fractions demonstrated significant and concentration-dependent antioxidant potential. In general, among fractions aqueous fraction exhibited a greater anti-arthritic effect. Phytochemical analysis of aqueous fraction confirmed the presence of flavonoids and glycosides, 215.29 mgGAE/ml phenolic content and 633.03 μgQE/ml flavonoid content. Thus, we suggest Clematis orientalis as a potent strategy for the treatment of rheumatoid arthritis.
东方铁线莲长期以来一直被用作治疗关节炎的民族药物。本研究旨在评估东方铁线莲治疗大鼠多发性关节炎的疗效。在 CFA 免疫后，每天口服给予 200mg/kg 的水醇提取物和馏分（己烷、丁醇和水）28 天。评估爪肿胀、爪直径、关节炎评分、体重、血液学参数、踝关节的放射学和组织学分析。此外，通过 RT-PCR 和 ELISA 测量各种炎症标志物的水平。DPPH 和还原能力测定用于评估抗氧化能力。进行了定性植物化学分析、总酚和类黄酮含量的测定。水醇提取物和馏分显著（p < 0.001）降低爪体积、爪厚度和关节炎评分，并显著防止体重减轻以及血液学参数的异常改变，与关节炎对照组相比。X 射线和组织学检查显示治疗大鼠踝关节无明显结构变化。IL-1β、TNF-α、IL-6、COX-2 和 NF-Kβ 的表达水平显著（p < 0.05-0.001）下调，并且在治疗动物中检测到 IL-4 和 IL-10 的水平显著增加。不同治疗组动物 TNF-α和 PGE2 的过度产生得到了显著预防。水醇提取物及其馏分表现出显著的、浓度依赖性的抗氧化潜力。总的来说，在馏分中，水馏分表现出更强的抗关节炎作用。水馏分的植物化学分析证实了类黄酮和糖苷、215.29mgGAE/ml 酚含量和 633.03μgQE/ml 类黄酮含量的存在。因此，我们认为东方铁线莲是治疗类风湿关节炎的有效策略。
