Gut Paweł, Czarnywojtek Agata, Ziemnicka Katarzyna, Waligórska-Stachura Joanna, Sawicka-Gutaj Nadia, Bączyk Maciej, Fischbach Jakub, Woliński Kosma, Wrotkowska Elżbieta, Ruchała Marek
Department of Endocrinology, Metabolism and Internal Medicine, Poznań University of Medical Sciences, Poznań, Poland.
Cent Eur J Immunol. 2018;43(4):428-433. doi: 10.5114/ceji.2018.81346. Epub 2018 Dec 31.
Diabetes insipidus is a disorder resulting from insufficient action of vasopressin (ADH) characterized by excretion of highly diluted urine in large amounts. Idiopathic diabetes insipidus is associated with the presence of both autoantibodies against ADH-secreting neurons and pituitary autoantibodies. The aim of the present study was to evaluate the occurrence of autoantibodies against the pituitary microsomal fraction. The study included 33 sera of diabetes insipidus patients and 10 control sera obtained from 10 healthy persons. In all patients the secretion of pituitary hormones and thyroid autoantibodies was assessed. Human pituitaries were obtained during autopsy and homogenized in 0.01 mol/l pH 7.4 phosphate buffer. In addition, for the autoantibody evaluation, the electrophoretic method of separation in polyacrylamide gel and western blot were employed. Among the 33 subjects, in 23 patients the presence of autoantibodies against the pituitary was shown. Sera of 15 patients reacted with the pituitary microsomal fraction protein of 55 kDa. In other cases, 10 sera reacted with the pituitary antigen of 67 kDa. In addition, 5 sera reacted with the 60 kDa antigen, 5 sera with 52 kDa protein, 3 sera with 105 kDa protein, 3 sera with the 97 kDa antigen and 2 sera with pituitary antigen of 92 kDa weight. In our study, based on the immunoblotting method, we observed that pituitary autoantibodies against 55, 60 and 67 kDa antigens occurred frequently.
尿崩症是一种由于抗利尿激素（ADH）作用不足导致的疾病，其特征是大量排出高度稀释的尿液。特发性尿崩症与抗ADH分泌神经元自身抗体和垂体自身抗体的存在有关。本研究的目的是评估抗垂体微粒体部分自身抗体的发生情况。该研究包括33例尿崩症患者的血清和10例健康人提供的10份对照血清。对所有患者评估垂体激素分泌和甲状腺自身抗体。在尸检期间获取人垂体，并在0.01 mol/l pH 7.4的磷酸盐缓冲液中匀浆。此外，为了评估自身抗体，采用了聚丙烯酰胺凝胶电泳分离法和蛋白质印迹法。在33名受试者中，23例患者显示存在抗垂体自身抗体。15例患者的血清与55 kDa的垂体微粒体部分蛋白发生反应。在其他情况下，10份血清与67 kDa的垂体抗原发生反应。此外，5份血清与60 kDa抗原发生反应，5份血清与52 kDa蛋白发生反应，3份血清与105 kDa蛋白发生反应，3份血清与97 kDa抗原发生反应，2份血清与92 kDa的垂体抗原发生反应。在我们的研究中，基于免疫印迹法，我们观察到针对55、60和67 kDa抗原的垂体自身抗体频繁出现。
