Chen Wen-Chi, Chou Tzu-Yang, Chen Huey-Yi, Yang You-Rong, Man Kee-Ming, Tsai Ming-Yen, Chen Yung-Hsiang
Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan.
Departments of Urology, Obstetrics and Gynecology, and Medical Research, China Medical University Hospital, Taichung, Taiwan.
Evid Based Complement Alternat Med. 2019 Jan 20;2019:1408979. doi: 10.1155/2019/1408979. eCollection 2019.
Traditional Chinese medicine (TCM) has been prescribed for the treatment of stone disease for thousands of years. (Danshen) was previously shown to have potential for treatment of stone disease in animal and clinical studies. In this study, we further studied the antiurolithiasis effect of Danshen in a fly model. Wild-type male CS flies were used in this study, with 0.25% ethylene glycol (EG) as a lithogenic agent. 2% potassium citrate (K-citrate) was the positive control agent for prevention (all agents added at the start of experiment) and treatment (drugs added after 2-week addition of lithogenic agent) studies compared with 15, 30, and 60 g/ml of Danshen extract. In the prevention study, both 2% K-citrate and Danshen (30 and 60 g/ml) significantly inhibited EG-induced calcium oxalate (CaOx) crystal formation. In the treatment study, only 2% K-citrate and high-dose of Danshen (60 g/ml) significantly inhibited EG-induced CaOx crystal formation. Survival analysis for EG with Danshen was compared with that for EG with K-citrate. The mean lifespan was significantly reduced by administration of EG, and the results in the Danshen group were similar to those in the control group. In conclusion, Danshen revealed both preventive and treatment effects on CaOx crystal formation in a fly model.
数千年来，中药一直被用于治疗结石病。丹参此前在动物和临床研究中已显示出治疗结石病的潜力。在本研究中，我们在果蝇模型中进一步研究了丹参的抗结石作用。本研究使用野生型雄性CS果蝇，以0.25%的乙二醇（EG）作为致石剂。与15、30和60微克/毫升的丹参提取物相比，2%的柠檬酸钾（K-柠檬酸盐）是预防（所有试剂在实验开始时添加）和治疗（在添加致石剂2周后添加药物）研究的阳性对照剂。在预防研究中，2%的K-柠檬酸盐和丹参（30和60微克/毫升）均显著抑制EG诱导的草酸钙（CaOx）晶体形成。在治疗研究中，只有2%的K-柠檬酸盐和高剂量的丹参（60微克/毫升）显著抑制EG诱导的CaOx晶体形成。对EG与丹参组的生存分析与EG与K-柠檬酸盐组的生存分析进行了比较。给予EG显著缩短了平均寿命，丹参组的结果与对照组相似。总之，丹参在果蝇模型中对CaOx晶体形成显示出预防和治疗作用。
