Dorazio Dominic, Kitch Douglas W, Utay Netanya S, Macatangay Bernard J, Landay Alan, Brown Todd, Bosch Ronald J, Pelger Alison L, Baum Jane E, Asaad Robert, Rodriguez Benigno, Lederman Michael M
Case Western Reserve University School of Medicine, University Hospitals Cleveland Medical Center, Cleveland, OH, USA.
Harvard TH Chan School of Public Health, Boston, Massachusetts, USA.
J Virus Erad. 2019 Jan 1;5(1):28-32. doi: 10.1016/S2055-6640(20)30274-0.
The relationship between lipid levels in plasma and inflammatory indices is complex and fatty meals alter plasma inflammatory markers in people with diabetes. There is interest in monitoring the effects of interventions on plasma inflammatory and coagulation elements in people with HIV, as they have been linked to risk for morbid outcomes and HIV persistence. Understanding the effects of feeding and time of specimen acquisition is important for the correct scheduling of clinical sampling.
We examined the effects of feeding on plasma inflammatory, coagulation and homeostatic indices among 24 non-diabetic people with HIV, with controlled viraemia and on antiretroviral therapy after fasting and then 1, 3 and 6 hours after ingesting a fatty meal, and also approximately 1 week later after fasting and after an isocaloric non-fatty meal. Plasma levels of IL-6, IL-7, IP-10, sCD14, sCD163, sTNFrII and D-dimer were monitored by immunoassay.
Fasting levels of all markers obtained approximately 1 week apart were significantly correlated (<0.001). Mild alterations in plasma concentrations of inflammatory markers were observed after feeding but geometric means varied more than 10% from baseline for only IL-6 and IL-7. Meal type was differentially associated with changes in plasma levels for IL-7 only. Antiretroviral treatment regimen, body mass index and changes in plasma triglyceride levels were not linked to post-feeding changes in these biomarkers.
These plasma inflammatory, coagulation and homeostatic indices are relatively stable at fasting and are only minimally affected by feeding or time of day. These findings will aid in the monitoring of inflammatory and homeostatic indices that may contribute to control of HIV expression and its persistence.
血浆脂质水平与炎症指标之间的关系复杂，并且高脂餐会改变糖尿病患者的血浆炎症标志物。人们对监测干预措施对HIV感染者血浆炎症和凝血因子的影响很感兴趣，因为这些因素与不良结局风险和HIV持续存在有关。了解进食和标本采集时间的影响对于正确安排临床采样至关重要。
我们研究了进食对24名非糖尿病HIV感染者血浆炎症、凝血和内稳态指标的影响，这些患者病毒血症得到控制且正在接受抗逆转录病毒治疗。在空腹状态下，以及摄入高脂餐后1、3和6小时，还有大约1周后空腹和摄入等热量非高脂餐后，对这些指标进行检测。通过免疫测定法监测血浆中白细胞介素-6（IL-6）、白细胞介素-7（IL-7）、干扰素诱导蛋白10（IP-10）、可溶性CD14（sCD14）、可溶性CD163（sCD163）、可溶性肿瘤坏死因子受体II（sTNFrII）和D-二聚体的水平。
间隔约1周获得的所有标志物的空腹水平显著相关（<０.００１）。进食后观察到血浆炎症标志物浓度有轻微变化，但仅白细胞介素-6和白细胞介素-7的几何平均数与基线相比变化超过10％。仅进食类型与白细胞介素-7的血浆水平变化存在差异关联。抗逆转录病毒治疗方案、体重指数和血浆甘油三酯水平的变化与进食后这些生物标志物的变化无关。
这些血浆炎症、凝血和内稳态指标在空腹时相对稳定，仅受进食或一天中的时间的轻微影响。这些发现将有助于监测可能有助于控制HIV表达及其持续存在的炎症和内稳态指标。
