通过激光镊子拉曼光谱对人视网膜色素上皮细胞中过氧化氢诱导的氧化应激进行无标记检测。
Label-free detection of hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells via laser tweezers Raman spectroscopy.
作者信息
Chen Yang, Wang ZhiQiang, Huang Yan, Feng ShangYuan, Zheng ZuCi, Liu XiuJie, Liu MengMeng
机构信息
Department of Laboratory Medicine, Fujian Medical University, Fuzhou 350004, China.
Department of Ophthalmology & Optometry, Fujian Medical University, Fuzhou 350004, China.
出版信息
Biomed Opt Express. 2019 Jan 9;10(2):500-513. doi: 10.1364/BOE.10.000500. eCollection 2019 Feb 1.
Abstract
Human retinal pigment epithelium cells under hydrogen peroxide-induced oxidative stress and a ligustrazine-based protective effect were investigated using laser tweezers Raman spectroscopy. Protein and lipid were significantly affected by oxidative damage, along with increased reactive oxygen species (ROS) level within cells. The effects of ligustrazine against the reaction of ROS with protein seemed to be able to inhibit such damages but were limited during the desamidization of amides, along with additional effect on nucleic acid base and DNA phosphoric acid skeleton. This work laid the basis for both understanding the molecular mechanisms of oxidative stress-induced injury and highlighting possible biomarkers in retinal diseases.
摘要
利用激光镊子拉曼光谱研究了过氧化氢诱导的氧化应激下的人视网膜色素上皮细胞以及川芎嗪的保护作用。蛋白质和脂质受到氧化损伤的显著影响，同时细胞内活性氧（ROS）水平升高。川芎嗪对ROS与蛋白质反应的影响似乎能够抑制此类损伤，但在酰胺脱酰胺化过程中作用有限，同时对核酸碱基和DNA磷酸骨架有额外影响。这项工作为理解氧化应激诱导损伤的分子机制以及突出视网膜疾病中可能的生物标志物奠定了基础。
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