Department of Ophthalmology, Huaihe Hospital, Henan University, Kaifeng, China.
J Cell Biochem. 2019 Mar;120(3):4514-4522. doi: 10.1002/jcb.27739. Epub 2018 Sep 30.
Oxidative stress in retinal pigment epithelium (RPE) cells may contribute to the progression of age-related macular degeneration. Thymoquinone (TQ), an active component derived from Nigella sativa, possesses antioxidative effect. However, the role of TQ in RPE cells under oxidative stress condition remains unclear. The present study aimed to examine the protective effect of TQ against hydrogen peroxide (H O )-induced oxidative stress in human RPE cells. Our results showed that TQ improved the cell viability and apoptosis in H O -induced ARPE cells. We also found that the levels of reactive oxygen species and malondialdehyde induced by H O were reduced after the pretreatment of TQ. In addition, the inhibitory effect of H O on the glutathione (GSH) level and superoxide dismutase activity was markedly attenuated by TQ pretreatment. Moreover, TQ enhanced the activation of Nrf2/heme oxygenase 1 (HO-1) signaling pathway in H O -induced ARPE cells. Knockdown of Nrf2 abolished the protective effect of TQ on H O -induced oxidative damage. These results suggested that TQ protected ARPE cells from H O -induced oxidative stress and apoptosis via the Nrf2/HO-1 signaling pathway.
视网膜色素上皮 (RPE) 细胞中的氧化应激可能导致年龄相关性黄斑变性的进展。百里香醌 (TQ) 是从黑种草中提取的一种具有抗氧化作用的活性成分。然而，TQ 在氧化应激条件下对 RPE 细胞的作用尚不清楚。本研究旨在探讨 TQ 对人 RPE 细胞过氧化氢 (H2O2) 诱导的氧化应激的保护作用。我们的结果表明，TQ 可提高 H2O2 诱导的 ARPE 细胞活力和细胞凋亡。我们还发现，TQ 预处理可降低 H2O2 诱导的活性氧和丙二醛水平。此外，TQ 预处理明显减弱了 H2O2 对谷胱甘肽 (GSH) 水平和超氧化物歧化酶活性的抑制作用。此外，TQ 增强了 H2O2 诱导的 ARPE 细胞中 Nrf2/血红素加氧酶 1 (HO-1) 信号通路的激活。Nrf2 敲低消除了 TQ 对 H2O2 诱导的氧化损伤的保护作用。这些结果表明，TQ 通过 Nrf2/HO-1 信号通路保护 ARPE 细胞免受 H2O2 诱导的氧化应激和细胞凋亡。
