Truncated, strong inducible promoter P from .

In this study, collagen -like protein (MCL1) promoter from was analysed and truncated into different sizes through series of targeted and random deletions based on the presence of various transcription factor-binding sites. Synthetic Green Fluorescent Protein (sGFP) was being utilized as a reporter gene to study the relative expression driving capability of unmodified and truncated promoters. Conserved promoter sequence analysis revealed similarity between the paralogous promoters from and . sGFP expression in the haemolymph was directed with the help of signal peptide sequence. Deleting the promoter region from - 2764 to - 1583 bp increases the promoter (P) activity by twofolds, while deletions of the regions upstream of - 1150 bp and - 840 bp caused a decrease of sGFP expression level (80% and 70%, respectively). Transcriptional binding sites predicted for the deleted region revealed the loss of upstream repressing sequences such as Matalpha2 along with ROX1 and Rap1 repressor-binding sites located - 2234 bp, - 1754 bp and - 1724 bp from the TSS. Compared with Pwild type (2.7 kbp), P-1583 bp had a shorter sequence and showed statistically significant expression in . This study introduces a highly efficient strong inducible promoter for over-expression of target genes in .