Shakeel Faiyaz, Alam Prawez, Anwer Md Khalid, Alanazi Saleh A, Alsarra Ibrahim A, Alqarni Mohammed H
1Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451 Saudi Arabia.
2Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj, 11942 Saudi Arabia.
3 Biotech. 2019 Mar;9(3):82. doi: 10.1007/s13205-019-1630-y. Epub 2019 Feb 14.
The objective of this work was to characterize and evaluate wound healing potential of oil (PO) via self-nanoemulsifying drug delivery system (SNEDDS) in comparison with standard gentamycin. PO loaded SNEDDS was prepared by low energy emulsification technique and characterized for thermodynamic stability, self-emulsification power and various physico-chemical parameters. An optimal formula of PO SNEDDS was subjected to wound healing evaluation, collagen determination and histomorphological examination in female Wistar rats as compared with pure PO and standard antibiotic/gentamycin. An optimal formula of PO SNEDDS showed significant wound healing effects in Wistar female rats in comparison with pure PO. However, wound healing effects of optimized SNEDDS were comparable with standard gentamycin. An optimized formulation also indicated significant enhancement in collagen content (0.82 mg/g) in comparison with pure PO (0.53 mg/g) and negative control (0.33 mg/g). While, the collagen content of SNEDDS (0.82 mg/g) treated rats were comparable with standard gentamycin treated animals (0.98 mg/g). Histopathological examinations of optimized SNEDDS treated animals showed no signs of inflammatory cells which indicated that prepared SNEDDS was safe and nontoxic to rats. The results obtained in this work showed the potential application of SNEDDS in enhancement of the wound healing activity of PO upon oral administration.
本研究的目的是通过自纳米乳化药物递送系统（SNEDDS）来表征和评估油（PO）的伤口愈合潜力，并与标准庆大霉素进行比较。采用低能乳化技术制备了负载PO的SNEDDS，并对其热力学稳定性、自乳化能力和各种物理化学参数进行了表征。将PO SNEDDS的最佳配方与纯PO和标准抗生素/庆大霉素相比，在雌性Wistar大鼠中进行伤口愈合评估、胶原蛋白测定和组织形态学检查。与纯PO相比，PO SNEDDS的最佳配方在Wistar雌性大鼠中显示出显著的伤口愈合效果。然而，优化后的SNEDDS的伤口愈合效果与标准庆大霉素相当。与纯PO（0.53 mg/g）和阴性对照（0.33 mg/g）相比，优化后的配方还显示胶原蛋白含量显著提高（0.82 mg/g）。同时，SNEDDS（0.82 mg/g）处理的大鼠的胶原蛋白含量与标准庆大霉素处理的动物（0.98 mg/g）相当。对优化后的SNEDDS处理的动物进行组织病理学检查，未发现炎症细胞迹象，这表明所制备的SNEDDS对大鼠安全无毒。本研究获得的结果表明，SNEDDS在口服给药时增强PO的伤口愈合活性方面具有潜在应用价值。
