1 College of Enology, Northwest A&F University, Yangling, China.
2 State Key Laboratory of Crop Stress Biology in Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, China.
DNA Cell Biol. 2019 Apr;38(4):333-340. doi: 10.1089/dna.2018.4271. Epub 2019 Feb 25.
F-box proteins are a core component of Skp1-Cul1-F-box (SCF) ubiquitin/ligase complexes and are involved in a lot of cellular processes in yeasts. However, the current knowledge of the molecular evolution of the F-box gene family in yeasts remains unclear. In this study, 136 F-box genes were identified in 10 yeast species of the Saccharomycetaceae. In addition to the F-box domain, the other six domains were identified in these F-box proteins. The evolutionary history of F-box gene numbers in 10 Saccharomycetaceae yeasts was reconstructed. Whole-genome duplication, interspersed repeats, and gene loss events were inferred. These events contributed to F-box gene number variation in the 10 yeast species. Eighty-seven and 33 positively selected sites were detected in program Selecton and Datamonkey web-server, respectively. Three of them were considered the significant positively selected sites, and 23 of them had changed radically in amino acid properties by using TreeSAAP. We investigated F-box gene number variation and underlying mechanisms, and selection patterns, all of which were beneficial to deeply understand genome evolution and figure out the function of the F-box proteins.
F-box 蛋白是 Skp1-Cul1-F-box（SCF）泛素/连接酶复合物的核心组成部分，参与酵母中的许多细胞过程。然而，目前对于酵母中 F-box 基因家族的分子进化的了解仍不清楚。在这项研究中，从酿酒酵母科的 10 种酵母中鉴定出了 136 个 F-box 基因。除了 F-box 结构域，这些 F-box 蛋白还鉴定出了其他六个结构域。重建了 10 种酿酒酵母科酵母中 F-box 基因数量的进化历史。推断出了全基因组复制、分散重复和基因丢失事件。这些事件导致了 10 种酵母中 F-box 基因数量的变化。程序 Selecton 和 Datamonkey web-server 分别检测到了 87 个和 33 个正选择位点。其中有 3 个被认为是显著的正选择位点，有 23 个在氨基酸性质上发生了根本变化，使用 TreeSAAP 进行了分析。我们研究了 F-box 基因数量的变化及其潜在机制和选择模式，所有这些都有助于深入了解基因组进化并确定 F-box 蛋白的功能。
