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孢粉学技术在巴基斯坦拉合尔地区不同草药市场同名掺假药物鉴别中的应用

Implication of palynological techniques for the authentication of adulterated drugs traded with the same name in different herbal markets of district Lahore, Pakistan.

作者信息

Ramzan Sehrish, Shaheen Shabnum, Hussain Khadim, Sharifi-Rad Javad, Khan Muhammad Asaf, Harun Nidaa, Jaffer Mehwish, Khan Farah

机构信息

Department of Botany, Lahore College For Women University, Lahore, Pakistan.

Department of Bioinformatics & Biotechnology, Government College University, Faisalabad, Pakistan.

出版信息

Microsc Res Tech. 2019 Jun;82(6):931-940. doi: 10.1002/jemt.23240. Epub 2019 Feb 25.

Abstract

Adulteration in traded medicinal plants is a significant issue nowadays and use of these adulterated medicinal plants can impose harmful impact to end user. However, this problem can be overcome by ensuring the identification of traded medicinal plants which are used in making different herbal medicines. In this regard, palynological markers are considered to be an important taxonomic tool in the identification of original medicinal plant from its adulterant. Hence this study attempted to provide particular reliable palynological markers for distinguishing selected medicinal plants from their adulterants, that is, Cinnamomum verum versus Canella winterana, Cinnamomum tamala versus Cinnamomum obtusifolium, Gymnema sylvestre versus Gymnema lactiferum, Artemisia maritima versus Artemisia absinthium, Achillea millefolium versus Adhatoda vasaka, Sphaeranthus indicus versus Sphaeranthus africanus, Averrhoa carambola versus Butea monosperma, and Morus nigra versus Morus alba. Results demonstrated great variations in multiple palynological characters between original medicinal plant and its adulterant such as in pollen size, shape, colpi length, exine, intine thickness, and fertility. In equatorial view, circular to spheroidal shape of pollen was found in A. millefolium while oblate shape was observed in A. vasaka. Similarly B. monosperma pollen was 34 μm, whereas pollen of its adulterant A. carambola was 21 μm. Moreover, colpi length of A. maritima was 11.8 μm, whereas 4.5 μm in A. absinthium. Hence it can be concluded that palynological characters are commendably helpful in identification of genuine medicinal plant from its adulterant.

摘要

如今,贸易中的药用植物掺假是一个重大问题,使用这些掺假的药用植物会对终端用户造成有害影响。然而,通过确保识别用于制作不同草药的贸易药用植物,可以克服这个问题。在这方面,孢粉学标记被认为是从其掺假物中识别原始药用植物的重要分类工具。因此,本研究试图提供特别可靠的孢粉学标记,以区分选定的药用植物与其掺假物,即真肉桂与冬香桂、锡兰肉桂与钝叶桂、匙羹藤与乳叶匙羹藤、滨海蒿与苦艾、蓍草与鸭嘴花、印度艾纳香与非洲艾纳香、杨桃与紫铆、黑桑与白桑。结果表明,原始药用植物与其掺假物之间在多个孢粉学特征上存在很大差异,如花粉大小、形状、沟长、外壁、内壁厚度和育性。在赤道面观中,蓍草的花粉呈圆形至球形,而鸭嘴花的花粉呈扁球形。同样,紫铆的花粉为34μm,而其掺假物杨桃的花粉为21μm。此外,滨海蒿的沟长为11.8μm,而苦艾的沟长为4.5μm。因此,可以得出结论,孢粉学特征在从掺假物中识别真正的药用植物方面非常有帮助。

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