Shaheen Shabnum, Jaffer Mehwish, Khan Farah, Hussain Khadim, Hanif Uzma, Younis Sidra, Ilyas Sobia, Ishtiaq Saiqa
Department of Plant Sciences, Lahore College for Women University, Lahore, Pakistan.
Bioinformatics & Biotechnology, Government College University, Faisalabad, Pakistan.
Microsc Res Tech. 2018 Dec;81(12):1397-1405. doi: 10.1002/jemt.23096. Epub 2018 Nov 5.
This study was carried out to identify 20 high valued medicinal plants of District Lahore, Punjab, Pakistan with the help of the palynological studies. The distinguishing characteristics for selected species were found by systematized methods using light microscopy and Scanning Electron Microscopy. In this study, important pollen qualitative and quantitative features of these medicinal species such as polar and equatorial outline and their diameter, P/E ratio, presence or absence of pore, sculpturing of pollen, exine thickness, intine thickness, intercellular distance of exine and intine, presence or absence of spines, length of spines, width of spines, presence or absence of colpi and length of colpi, were compared. Remarkable variations in these pollen characters have been observed. Pollen grains were usually circular-semicircular (Calendula officinalis), spheroidal (Convolvulus arvensis), bilobed and trilobed (Convolvulus arvensis), spiny, (Abutilon indicum), oblate (Terminalia chebula) and elongated (Euphorbia pilulifera). In polar view Conyza bonariensis exhibited the highest pollen size [195.9 (211.6-180.2) μm]. While in equatorial view Calendula officinalis and Euphorbia pilulifera had lowest value [22.5 (20-25) μm] whereas Carissa carandas [165 (140-179) μm] appeared to be the largest. Parthenium hysterophorus showed the highest P/E ratio, that is, 1.28. Colpi were reported in only 7 species. Most of the plants showed 100% fertility but Terminalia chebula showed the least value 35%. In the present, project the diversity in pollen morphology present pollen as a valuable taxonomic tool. It is also concluded that pollen features are helpful at the specific level as well as generic level and can provide us fruitful taxonomic solutions. RESEARCH HIGHLIGHTS: Palynological flora of the medicinal plant species of district Lahore, Pakistan is investigated first time on the basis of LM and SEM and the flora is found very significant for taxonomic investigations.
本研究旨在借助孢粉学研究,鉴定巴基斯坦旁遮普省拉合尔地区的20种高价值药用植物。通过使用光学显微镜和扫描电子显微镜的系统化方法,找出所选物种的显著特征。在本研究中,比较了这些药用植物重要的花粉定性和定量特征,如极面和赤道面轮廓及其直径、P/E比、孔的有无、花粉纹饰、外壁厚度、内壁厚度、外壁与内壁的细胞间距离、刺的有无、刺的长度、刺的宽度、沟的有无及沟的长度。观察到这些花粉特征存在显著差异。花粉粒通常为圆形 - 半圆形(金盏花)、球形(田旋花)、双叶和三叶(田旋花)、具刺(苘麻)、扁球形(诃子)和长形(小飞扬草)。在极面观中,阿根廷蟛蜞菊的花粉尺寸最大[195.9 (211.6 - 180.2) μm]。而在赤道面观中,金盏花和小飞扬草的值最低[22.5 (20 - 25) μm],而刺黄果茄[165 (140 - 179) μm]似乎最大。银胶菊的P/E比最高,即1.28。仅在7个物种中发现有沟。大多数植物的育性为100%,但诃子的育性最低,为35%。在本项目中,花粉形态的多样性表明花粉是一种有价值的分类工具。还得出结论,花粉特征在种级和属级水平上都有帮助,可为我们提供有效的分类学解决方案。研究亮点:首次基于光学显微镜和扫描电子显微镜对巴基斯坦拉合尔地区药用植物物种的孢粉植物区系进行研究,发现该植物区系对分类学研究具有重要意义。