Chang Andrew L, Hole Camaron R, Doering Tamara L
Washington University in St. Louis, St. Louis, Missouri.
Curr Protoc Microbiol. 2019 Jun;53(1):e79. doi: 10.1002/cpmc.79. Epub 2019 Feb 25.
Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningoencephalitis, which kills 200,000 individuals worldwide each year. It is ubiquitous in the environment and is first inhaled into the lungs of the host, where it is taken up by phagocytes. The interaction of these fungal cells with host phagocytes, therefore, is a critical step in the pathogenesis of this disease. One characteristic of this initial step in host-pathogen interactions is the avidity with which fungal cells are taken up by phagocytes, described by the phagocytic index. In this chapter, we detail a high-throughput method of directly assessing the phagocytic index of fungal cells using an imaging-based paradigm. By automating image collection and processing, this method permits rapid assessment of this critical host interaction. © 2019 by John Wiley & Sons, Inc.
新型隐球菌是一种机会性真菌病原体，可引起脑膜脑炎，每年在全球导致20万人死亡。它在环境中普遍存在，首先被吸入宿主肺部，在那里被吞噬细胞摄取。因此，这些真菌细胞与宿主吞噬细胞的相互作用是该疾病发病机制中的关键步骤。宿主 - 病原体相互作用这一初始步骤的一个特征是吞噬细胞摄取真菌细胞的亲和力，用吞噬指数来描述。在本章中，我们详细介绍一种基于成像范式直接评估真菌细胞吞噬指数的高通量方法。通过自动化图像采集和处理，该方法能够快速评估这一关键的宿主相互作用。© 2019约翰威立父子公司版权所有
