Nigam Kumud, Sanyal Somali, Gupta Shalini, Gupta Om Prakash, Mahdi Abbas Ali, Bhatt M L B
Department of Oral Pathology and Microbiology, King George’s Medical University, Lucknow, India. Email:
Asian Pac J Cancer Prev. 2019 Feb 26;20(2):345-354. doi: 10.31557/APJCP.2019.20.2.345.
Background: The aim of this study was to evaluate any association between CYP1A1 (T6235C and C4887A, A4889G) gene polymorphisms and the risk of oral pre-cancer and cancer. Methods: In the present study, 250 patients with oral pre-cancer and/or cancer and 250 healthy controls were genotyped for CYP1A1 T6235C, C4887A and A4889G polymorphisms by the PCR-RFLP method. Results: None of the CYP1A1 polymorphisms were associated with the risk of either oral cancer or pre cancer. Nor were any links with clinical parameters of oral cancer found. However, among the consumers of areca nut/pan masala the TC, CA and AG genotypes respectively for the CYP1A1 T6235C,C4887Aand A4889G polymorphisms were significantly more frequent in controls compared to cases (p values for cases vs. controls of 0.0032, 0.0019 and 0.0009, respectively). Similarly, compared to the haplotype TCA, TAG constituted by CYP1A1 T6235C and C4887A and A4889G was more common in controls (6.88%) than in cases (4.07%). Conclusion: Our results suggest that genotypes regarding CYP1A1 polymorphisms may modulate the risk of oral cancer and pre-cancer among the areca nut/pan masala consumers. The haplotype may also exert an influence in our north Indian population.
本研究旨在评估CYP1A1（T6235C、C4887A、A4889G）基因多态性与口腔癌前病变及癌症风险之间的任何关联。方法：在本研究中，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对250例口腔癌前病变和/或癌症患者以及250名健康对照者进行CYP1A1 T6235C、C4887A和A4889G多态性基因分型。结果：CYP1A1的任何多态性均与口腔癌或癌前病变的风险无关。也未发现与口腔癌临床参数有任何关联。然而，在食用槟榔/槟榔混合物的人群中，CYP1A1 T6235C、C4887A和A4889G多态性的TC、CA和AG基因型在对照者中的频率分别显著高于病例组（病例组与对照组的p值分别为0.0032、0.0019和0.0009）。同样，与单倍型TCA相比，由CYP1A1 T6235C、C4887A和A4889G构成的单倍型TAG在对照者中（6.88%）比在病例组中（4.07%）更常见。结论：我们的结果表明，CYP1A1多态性的基因型可能会调节食用槟榔/槟榔混合物人群患口腔癌和癌前病变的风险。单倍型在我们的北印度人群中也可能产生影响。
