Xie Changan, Goodman Charles, Dinno Mumtaz, Li Yong-Qing
Opt Express. 2004 Dec 13;12(25):6208-14. doi: 10.1364/opex.12.006208.
We report on real-time Raman spectroscopic studies of optically trapped living cells and organelles using an inverted confocal laser-tweezers-Raman-spectroscopy (LTRS) system. The LTRS system was used to hold a single living cell in a physiological solution or to hold a functional organelle within a living cell and consequently measured its Raman spectra. We have measured the changes in Raman spectra of a trapped yeast cell as the function of the temperature of the bathing solution and studied the irreversible cell degeneration during the heat denaturation. In addition, we measured the in-vitro Raman spectra of the nuclei within living pine cells and B. sporeformer, Strep. salivarius, and E. coli bacteria suspended in solution and showed the possibility of using LTRS system as a sensor for rapid identification of microbes in a fluid.
我们报道了使用倒置共焦激光镊子拉曼光谱（LTRS）系统对光学捕获的活细胞和细胞器进行的实时拉曼光谱研究。LTRS系统用于在生理溶液中捕获单个活细胞，或在活细胞内捕获功能性细胞器，并由此测量其拉曼光谱。我们测量了捕获的酵母细胞的拉曼光谱随浴液温度的变化，并研究了热变性过程中细胞的不可逆退化。此外，我们测量了悬浮在溶液中的活松树细胞、芽孢杆菌、唾液链球菌和大肠杆菌内细胞核的体外拉曼光谱，并展示了使用LTRS系统作为快速识别流体中微生物的传感器的可能性。
