Vollkommer Tobias, Henningsen Anders, Friedrich Reinhard E, Felthaus Oliver Heinrich, Eder Fabian, Morsczeck Christian, Smeets Ralf, Gehmert Sebastian, Gosau Martin
Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
In Vivo. 2019 Mar-Apr;33(2):337-347. doi: 10.21873/invivo.11479.
BACKGROUND/AIM: High-density porous polyethylene (PP) offers possibilities for reconstruction in craniofacial surgery. The purpose of this study was to evaluate the extent of inflammation and foreign body reactions to PP in vitro and in vivo.
Cell attachment, proliferation and expression of inflammatory cytokines were assessed using murine macrophages (RAW 264.7) on two different PP materials in vitro. In vivo, Balb/c mice received PP implants at their dorsum. After sacrifice, samples were analyzed histologically and real-time PCR was used to assess expression of inflammatory cytokines.
Cells showed a significantly decreased proliferation (p<0.001) after 48 h and a significantly increased expression of TNF-α (p<0.05) at 24, 48 and 72 h. All animals showed foreign body cell reactions and signs of chronic inflammation. Expression of all but one of the investigated cytokines dropped to non-significant levels after an initial increase.
Application of porous polyethylene can cause local chronic inflammatory reactions. Although clinical application seems to be immunologically safe, indication and risks should be evaluated carefully when using PP implants.
背景/目的：高密度多孔聚乙烯（PP）为颅面外科手术重建提供了可能。本研究的目的是评估体外和体内对PP的炎症程度及异物反应。
在体外，使用小鼠巨噬细胞（RAW 264.7）评估两种不同PP材料上的细胞附着、增殖及炎性细胞因子的表达。在体内，将PP植入Balb/c小鼠背部。处死后，对样本进行组织学分析，并使用实时PCR评估炎性细胞因子的表达。
48小时后细胞增殖显著降低（p<0.001），在24、48和72小时时肿瘤坏死因子-α（TNF-α）表达显著增加（p<0.05）。所有动物均出现异物细胞反应和慢性炎症迹象。除一种研究的细胞因子外，所有细胞因子的表达在最初增加后均降至无显著意义的水平。
多孔聚乙烯的应用可引起局部慢性炎症反应。尽管临床应用在免疫学上似乎是安全的，但使用PP植入物时应仔细评估适应证和风险。
