Institute for Clinical and Experimental Surgery, University of Saarland, D-66421, Homburg/Saar, Germany.
Eur Cell Mater. 2010 Feb 26;19:107-16. doi: 10.22203/ecm.v019a11.
Porous polyethylene (Medpor) is an alloplastic biomaterial, which is commonly used in plastic and reconstructive surgery. In the present study, we analyzed the effect of perioperative steroid administration on the inflammatory and angiogenic host tissue response to implanted Medpor. For this purpose, Medpor was implanted into the dorsal skinfold chamber of prednisolone-treated and vehicle-treated (control) balb/c mice and analyzed by means of intravital fluorescence microscopy over a 14-day period. Incorporation of the implants was evaluated by histology. An aortic ring assay and Western blot analyses were performed to determine in vitro the effect of prednisolone on angiogenesis. Implantation of Medpor did not induce a leukocytic inflammatory host tissue response. However, in prednisolone-treated and control animals giant cells could be detected at the interface between the implants and the surrounding granulation tissue as a typical indicator for a chronic foreign body reaction. Interestingly, perioperative prednisolone administration inhibited vascularisation of the implants, as indicated by a significantly decreased functional density of newly developing capillary blood vessels. Accordingly, prednisolone suppressed in vitro endothelial sprouting and tube formation in the aortic ring assay and reduced proliferating cell nuclear antigen (PCNA), Tie2, vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP)-9 expression of murine endothelioma cells. In conclusion, prednisolone treatment inhibits the early vascularisation of Medpor implants due to direct inhibition of distinct angiogenic mechanisms. Therefore, perioperative steroid therapy should be avoided in case of Medpor implantation to achieve a rapid incorporation of the biomaterial at the implantation site.
多孔聚乙烯（Medpor）是一种同种异体生物材料，常用于整形和重建外科。在本研究中，我们分析了围手术期类固醇给药对植入 Medpor 后炎症和血管生成宿主组织反应的影响。为此，将 Medpor 植入泼尼松龙处理和载体处理（对照）balb/c 小鼠的背部皮肤囊腔，并通过活体荧光显微镜在 14 天内进行分析。通过组织学评估植入物的整合情况。进行主动脉环测定和 Western blot 分析，以确定泼尼松龙对血管生成的体外影响。植入 Medpor 不会引起白细胞炎症性宿主组织反应。然而，在泼尼松龙处理和对照动物中，在植入物与周围肉芽组织之间的界面处可以检测到大细胞，这是慢性异物反应的典型标志。有趣的是，围手术期泼尼松龙给药抑制了植入物的血管生成，这表现为新形成的毛细血管功能性密度显著降低。因此，泼尼松龙在主动脉环测定中抑制了内皮细胞的发芽和管形成，并降低了增殖细胞核抗原（PCNA）、Tie2、血管内皮生长因子（VEGF）和基质金属蛋白酶（MMP）-9 的表达在小鼠内皮瘤细胞中。总之，泼尼松龙治疗抑制了 Medpor 植入物的早期血管生成，这是由于直接抑制了不同的血管生成机制。因此，为了在植入部位快速整合生物材料，应避免 Medpor 植入术中的围手术期类固醇治疗。
