唾液中的甘氨酸是岩藻黄质减轻AOM/DSS小鼠息肉形成和肿瘤微环境形成的重要预测指标。
Salivary Glycine Is a Significant Predictor for the Attenuation of Polyp and Tumor Microenvironment Formation by Fucoxanthin in AOM/DSS Mice.
作者信息
Terasaki Masaru, Masaka Saki, Fukada Chinami, Houzaki Mayu, Endo Tetsuya, Tanaka Takuji, Maeda Hayato, Miyashita Kazuo, Mutoh Michihiro
机构信息
School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan
Cancer Prevention Laboratories, Health Sciences University of Hokkaido, Hokkaido, Japan.
出版信息
In Vivo. 2019 Mar-Apr;33(2):365-374. doi: 10.21873/invivo.11483.
UNLABELLED
Background/ Aim: A high polar xanthophyll of Fucoxanthin (Fx) is abundantly contained in edible brown algae, and it has chemopreventive effects in mouse cancer models, however, the underlying mechanisms of these effects are not well understood. Thus, we aimed to investigate the effects of Fx on the tumor microenvironment in cancer model mice.
MATERIALS AND METHODS
We investigated the effect of Fx (30 mg/kg body weight) in a variety of cell types within the tumor microenvironment of α mouse preclinical colorectal cancer model and analyzed the mouse saliva in search of predictors for cancer chemopreventive effects.
RESULTS
Fx administration significantly decreased the number of colorectal polyps and tended to decrease colonic lesions compared to untreated control mice. In addition, Fx administration showed significantly lower numbers of colorectal cancer stem cells-like CD44/EpCAM cells, cancer-associated fibroblasts-like αSMA cells, tumor-associated macrophages-like and dendritic cells-like CD206 cells by 0.6-, 0.5- and 0.6-fold, respectively, compared to untreated control mice. Moreover, the treatment also showed significantly lower levels of salivary glycine by 0.5-fold.
CONCLUSION
Our results suggest that salivary glycine may be a predictor representing the chemopreventive effect of Fx in mice.
未标记
背景/目的：岩藻黄质（Fx）是一种高极性叶黄素，大量存在于可食用褐藻中，在小鼠癌症模型中具有化学预防作用，然而，这些作用的潜在机制尚不清楚。因此，我们旨在研究Fx对癌症模型小鼠肿瘤微环境的影响。
材料与方法
我们在α小鼠临床前结直肠癌模型的肿瘤微环境中研究了Fx（30mg/kg体重）对多种细胞类型的影响，并分析了小鼠唾液，以寻找癌症化学预防作用的预测指标。
结果
与未治疗的对照小鼠相比，给予Fx显著减少了结直肠息肉的数量，并倾向于减少结肠病变。此外，与未治疗的对照小鼠相比，给予Fx后，结直肠癌干细胞样CD44/EpCAM细胞、癌症相关成纤维细胞样αSMA细胞、肿瘤相关巨噬细胞样和树突状细胞样CD206细胞的数量分别显著降低了0.6倍、0.5倍和0.6倍。此外，该治疗还使唾液甘氨酸水平显著降低了0.5倍。
结论
我们的结果表明，唾液甘氨酸可能是代表Fx对小鼠化学预防作用的一个预测指标。
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